Abstract
T H17 cells are a lineage of CD4+ T cells that are critical for host defense and autoimmunity by expressing the cytokines IL-17A, IL-17F, and IL-22. A feature of T H17 cells at steady state is their ubiquitous presence in the lamina propria of the small intestine. The induction of these steady-state intestinal T H17 (sT H17) cells is dependent on the presence of the microbiota. However, the signaling pathway linking the microbiota to the development of intestinal sT H17 cells remains unclear. In this study, we show that IL-1β, but not IL-6, is induced by the presence of the microbiota in intestinal macrophages and is required for the induction of sT H17 cells. In the absence of IL-1β-IL-1R or MyD88 signaling, there is a selective reduction in the frequency of intestinal sT H17 cells and impaired production of IL-17 and IL-22. Myeloid differentiation factor 88-deficient (MyD88 -/-) and germ-free (GF) mice, but not IL-1R -/- mice, exhibit impairment in IL-1β induction. Microbiota-induced IL-1β acts directly on IL-1R-expressing T cells to drive the generation of sT H17 cells. Furthermore, administration of IL-1β into GF mice induces the development of retinoic acid receptor-related orphan receptor γt-expressing sT H17 cells in the small intestine, but not in the spleen. Thus, commensal-induced IL-1β production is a critical step for sT H17 differentiation in the intestine, which may have therapeutic implications for T H17-mediated pathologies.
| Original language | English |
|---|---|
| Pages (from-to) | 251-258 |
| Number of pages | 8 |
| Journal | Journal of Experimental Medicine |
| Volume | 209 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Feb |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Microbiota-induced IL-1β, but not IL-6, is critical for the development of steady-state T H17 cells in the intestine. / Shaw, Michael H.; Kamada, Nobuhiko; Kim, Yungi; Núñez, Gabriel.
In: Journal of Experimental Medicine, Vol. 209, No. 2, 02.2012, p. 251-258.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microbiota-induced IL-1β, but not IL-6, is critical for the development of steady-state T H17 cells in the intestine
AU - Shaw, Michael H.
AU - Kamada, Nobuhiko
AU - Kim, Yungi
AU - Núñez, Gabriel
PY - 2012/2
Y1 - 2012/2
N2 - T H17 cells are a lineage of CD4+ T cells that are critical for host defense and autoimmunity by expressing the cytokines IL-17A, IL-17F, and IL-22. A feature of T H17 cells at steady state is their ubiquitous presence in the lamina propria of the small intestine. The induction of these steady-state intestinal T H17 (sT H17) cells is dependent on the presence of the microbiota. However, the signaling pathway linking the microbiota to the development of intestinal sT H17 cells remains unclear. In this study, we show that IL-1β, but not IL-6, is induced by the presence of the microbiota in intestinal macrophages and is required for the induction of sT H17 cells. In the absence of IL-1β-IL-1R or MyD88 signaling, there is a selective reduction in the frequency of intestinal sT H17 cells and impaired production of IL-17 and IL-22. Myeloid differentiation factor 88-deficient (MyD88 -/-) and germ-free (GF) mice, but not IL-1R -/- mice, exhibit impairment in IL-1β induction. Microbiota-induced IL-1β acts directly on IL-1R-expressing T cells to drive the generation of sT H17 cells. Furthermore, administration of IL-1β into GF mice induces the development of retinoic acid receptor-related orphan receptor γt-expressing sT H17 cells in the small intestine, but not in the spleen. Thus, commensal-induced IL-1β production is a critical step for sT H17 differentiation in the intestine, which may have therapeutic implications for T H17-mediated pathologies.
AB - T H17 cells are a lineage of CD4+ T cells that are critical for host defense and autoimmunity by expressing the cytokines IL-17A, IL-17F, and IL-22. A feature of T H17 cells at steady state is their ubiquitous presence in the lamina propria of the small intestine. The induction of these steady-state intestinal T H17 (sT H17) cells is dependent on the presence of the microbiota. However, the signaling pathway linking the microbiota to the development of intestinal sT H17 cells remains unclear. In this study, we show that IL-1β, but not IL-6, is induced by the presence of the microbiota in intestinal macrophages and is required for the induction of sT H17 cells. In the absence of IL-1β-IL-1R or MyD88 signaling, there is a selective reduction in the frequency of intestinal sT H17 cells and impaired production of IL-17 and IL-22. Myeloid differentiation factor 88-deficient (MyD88 -/-) and germ-free (GF) mice, but not IL-1R -/- mice, exhibit impairment in IL-1β induction. Microbiota-induced IL-1β acts directly on IL-1R-expressing T cells to drive the generation of sT H17 cells. Furthermore, administration of IL-1β into GF mice induces the development of retinoic acid receptor-related orphan receptor γt-expressing sT H17 cells in the small intestine, but not in the spleen. Thus, commensal-induced IL-1β production is a critical step for sT H17 differentiation in the intestine, which may have therapeutic implications for T H17-mediated pathologies.
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UR - http://www.scopus.com/inward/citedby.url?scp=84863151799&partnerID=8YFLogxK
U2 - 10.1084/jem.20111703
DO - 10.1084/jem.20111703
M3 - Article
C2 - 22291094
AN - SCOPUS:84863151799
VL - 209
SP - 251
EP - 258
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 2
ER -