Microchip-based cellular biochemical systems for practical applications and fundamental research: From microfluidics to nanofluidics

Yan Xu, Kihoon Jang, Tadahiro Yamashita, Yo Tanaka, Kazuma Mawatari, Takehiko Kitamori

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

By combining cell technology and microchip technology, innovative cellular biochemical tools can be created from the microscale to the nanoscale for both practical applications and fundamental research. On the microscale level, novel practical applications taking advantage of the unique capabilities of microfluidics have been accelerated in clinical diagnosis, food safety, environmental monitoring, and drug discovery. On the other hand, one important trend of this field is further downscaling of feature size to the 10 1-10 3 nm scale, which we call extended-nano space. Extended-nano space technology is leading to the creation of innovative nanofluidic cellular and biochemical tools for analysis of single cells at the single-molecule level. As a pioneering group in this field, we focus not only on the development of practical applications of cellular microchip devices but also on fundamental research to initiate new possibilities in the field. In this paper, we review our recent progress on tissue reconstruction, routine cell-based assays on microchip systems, and preliminary fundamental method for single-cell analysis at the single-molecule level with integration of the burgeoning technologies of extended-nano space. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalAnalytical and bioanalytical chemistry
Volume402
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1
Externally publishedYes

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Keywords

  • Cell-based assay
  • DNA detection
  • Extended-nano space
  • Single cell
  • Single molecule
  • Tissue engineering

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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