TY - GEN
T1 - MICROCOAXIAL ELECTRODE FOR LOCAL NITRIC OXIDE MEASUREMENT IN BIOLOGICAL SYSTEMS
AU - Kitamura, Yoshiichiro
AU - Uzawa, Tomoyuki
AU - Oka, Kotaro
AU - Komai, Yutaka
AU - Ogawa, Hiroto
AU - Kobayashi, Hirosuke
AU - Takizawa, Naosada
AU - Tanishita, Kazuo
N1 - Funding Information:
This work was partially supported by "Research for the Future" program of Japan Society for the Promotion of Science (contract number JSPS-RFTF 96100102), the Asahi Glass Foundation, and Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Science, Sports and Culture in Japan.
Publisher Copyright:
© 1998 American Society of Mechanical Engineers (ASME). All rights reserved.
PY - 1998
Y1 - 1998
N2 - A new nitric oxide (NO) selective electrode was developed. The tip diameter of the NO electrode is less than 10 pm. A working and a reference electrodes are located coaxially at the tip of the NO electrode. A stable NO donor SNAP (S-nitroso-N-acetyl-DL-penicillamine) and a putative NO scavenger hemoglobin (Hb) was used to examine the response of the electrode. Considering the instantaneous concentration of NO secretion previously reported in biological systems, 0.1, 1 pM NO solution was used to calibrate the electrode. From the result of the test of the electrode with SNAP and Hb, we confirmed this microcoaxial NO electrode can detect NO released from SNAP directly and continuously. From the result of calibration of the electrode, good linearity was examined in sub-molar concentration range. It is expected to detect local NO concentration in biological systems with a high space resolution.
AB - A new nitric oxide (NO) selective electrode was developed. The tip diameter of the NO electrode is less than 10 pm. A working and a reference electrodes are located coaxially at the tip of the NO electrode. A stable NO donor SNAP (S-nitroso-N-acetyl-DL-penicillamine) and a putative NO scavenger hemoglobin (Hb) was used to examine the response of the electrode. Considering the instantaneous concentration of NO secretion previously reported in biological systems, 0.1, 1 pM NO solution was used to calibrate the electrode. From the result of the test of the electrode with SNAP and Hb, we confirmed this microcoaxial NO electrode can detect NO released from SNAP directly and continuously. From the result of calibration of the electrode, good linearity was examined in sub-molar concentration range. It is expected to detect local NO concentration in biological systems with a high space resolution.
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U2 - 10.1115/imece1998-0187
DO - 10.1115/imece1998-0187
M3 - Conference contribution
AN - SCOPUS:85124383514
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
SP - 387
EP - 388
BT - Advances in Bioengineering
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 1998 International Mechanical Engineering Congress and Exposition, IMECE 1998
Y2 - 15 November 1998 through 20 November 1998
ER -