Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source

Takeshi Ito, Masayuki Kunimatsu, Satoru Kaneko, Seishiro Ohya, Koji Suzuki

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We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (μ-DMFC, i.d. 500 μm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two μ-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a μ-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.

Original languageEnglish
Pages (from-to)1725-1730
Number of pages6
JournalAnalytical chemistry
Issue number4
Publication statusPublished - 2007 Feb 15


ASJC Scopus subject areas

  • Analytical Chemistry

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