Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source

Takeshi Ito, Masayuki Kunimatsu, Satoru Kaneko, Seishiro Ohya, Koji Suzuki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (μ-DMFC, i.d. 500 μm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two μ-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a μ-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.

Original languageEnglish
Pages (from-to)1725-1730
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number4
DOIs
Publication statusPublished - 2007 Feb 15

Fingerprint

Direct methanol fuel cells (DMFC)
Microfluidics
Dams
Glucose
Glucose Oxidase
Microchannels
Hydrogen Peroxide
Disposability
Detectors
Oxidation
Microelectrodes
Substrates
Fuel cells
Electrodes
Electric potential
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source. / Ito, Takeshi; Kunimatsu, Masayuki; Kaneko, Satoru; Ohya, Seishiro; Suzuki, Koji.

In: Analytical Chemistry, Vol. 79, No. 4, 15.02.2007, p. 1725-1730.

Research output: Contribution to journalArticle

Ito, Takeshi ; Kunimatsu, Masayuki ; Kaneko, Satoru ; Ohya, Seishiro ; Suzuki, Koji. / Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source. In: Analytical Chemistry. 2007 ; Vol. 79, No. 4. pp. 1725-1730.
@article{f27236d8fd2a4450b408f4d561c86448,
title = "Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source",
abstract = "We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (μ-DMFC, i.d. 500 μm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two μ-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a μ-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.",
author = "Takeshi Ito and Masayuki Kunimatsu and Satoru Kaneko and Seishiro Ohya and Koji Suzuki",
year = "2007",
month = "2",
day = "15",
doi = "10.1021/ac0618167",
language = "English",
volume = "79",
pages = "1725--1730",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source

AU - Ito, Takeshi

AU - Kunimatsu, Masayuki

AU - Kaneko, Satoru

AU - Ohya, Seishiro

AU - Suzuki, Koji

PY - 2007/2/15

Y1 - 2007/2/15

N2 - We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (μ-DMFC, i.d. 500 μm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two μ-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a μ-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.

AB - We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (μ-DMFC, i.d. 500 μm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two μ-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a μ-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.

UR - http://www.scopus.com/inward/record.url?scp=33847225243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847225243&partnerID=8YFLogxK

U2 - 10.1021/ac0618167

DO - 10.1021/ac0618167

M3 - Article

C2 - 17297980

AN - SCOPUS:33847225243

VL - 79

SP - 1725

EP - 1730

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 4

ER -