Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids

Hiroki Ota, Taiga Kodama, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells are collected to form a spheroid in chambers by micro-rotational flow in two minutes. The developed array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations less than 19 % by varying the cell density of the medium without altering the device geometry. Using the developed perfusion system we experimentally observed that the sizes of formed spheroids remained constant for two days and that the detoxification enzyme, CYP1A1, activities increased.

Original languageEnglish
Title of host publication2010 International Symposium on Micro-NanoMechatronics and Human Science
Subtitle of host publicationFrom Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
Pages362-366
Number of pages5
DOIs
Publication statusPublished - 2010 Dec 1
Event21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 - Nagoya, Japan
Duration: 2010 Nov 72010 Nov 10

Publication series

Name2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010

Other

Other21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
CountryJapan
CityNagoya
Period10/11/710/11/10

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

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