Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids

Hiroki Ota; Taiga Kodama; Norihisa Miki

doi:10.1109/MHS.2010.5669519

Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids

Hiroki Ota, Taiga Kodama, Norihisa Miki

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells are collected to form a spheroid in chambers by micro-rotational flow in two minutes. The developed array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations less than 19 % by varying the cell density of the medium without altering the device geometry. Using the developed perfusion system we experimentally observed that the sizes of formed spheroids remained constant for two days and that the detoxification enzyme, CYP1A1, activities increased.

Original language	English
Title of host publication	2010 International Symposium on Micro-NanoMechatronics and Human Science
Subtitle of host publication	From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
Pages	362-366
Number of pages	5
DOIs	https://doi.org/10.1109/MHS.2010.5669519
Publication status	Published - 2010 Dec 1
Event	21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 - Nagoya, Japan Duration: 2010 Nov 7 → 2010 Nov 10

Publication series

Name	2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010

Other

Other	21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
Country/Territory	Japan
City	Nagoya
Period	10/11/7 → 10/11/10

ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering

Access to Document

10.1109/MHS.2010.5669519

Cite this

Ota, H., Kodama, T., & Miki, N. (2010). Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. In 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010 (pp. 362-366). [5669519] (2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010). https://doi.org/10.1109/MHS.2010.5669519

Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. / Ota, Hiroki; Kodama, Taiga; Miki, Norihisa.

2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010. 2010. p. 362-366 5669519 (2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ota, H, Kodama, T & Miki, N 2010, Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. in 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010., 5669519, 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010, pp. 362-366, 21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010, Nagoya, Japan, 10/11/7. https://doi.org/10.1109/MHS.2010.5669519

Ota H, Kodama T, Miki N. Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. In 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010. 2010. p. 362-366. 5669519. (2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010). doi: 10.1109/MHS.2010.5669519

Ota, Hiroki ; Kodama, Taiga ; Miki, Norihisa. / Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids. 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010. 2010. pp. 362-366 (2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010).

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abstract = "We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells are collected to form a spheroid in chambers by micro-rotational flow in two minutes. The developed array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations less than 19 % by varying the cell density of the medium without altering the device geometry. Using the developed perfusion system we experimentally observed that the sizes of formed spheroids remained constant for two days and that the detoxification enzyme, CYP1A1, activities increased.",

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Microfluidic experimental array using micro-rotation flow for producing size-controlled three-dimensional spheroids

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