Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids

Hiroki Ota, Taiga Kodama, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells are collected to form a spheroid in chambers by micro-rotational flow in two minutes. The developed array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations less than 19 % by varying the cell density of the medium without altering the device geometry. Using the developed perfusion system we experimentally observed that the sizes of formed spheroids remained constant for two days and that the detoxification enzyme, CYP1A1, activities increased.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages2080-2082
Number of pages3
Volume3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Fingerprint

Rotational flow
Detoxification
Enzyme activity
Microfluidics
Geometry

Keywords

  • Hepatocyte
  • Micro-rotation
  • Microfluidics
  • Spheroid

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Ota, H., Kodama, T., & Miki, N. (2010). Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 3, pp. 2080-2082)

Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids. / Ota, Hiroki; Kodama, Taiga; Miki, Norihisa.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. p. 2080-2082.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ota, H, Kodama, T & Miki, N 2010, Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 3, pp. 2080-2082, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/10/3.
Ota H, Kodama T, Miki N. Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3. 2010. p. 2080-2082
Ota, Hiroki ; Kodama, Taiga ; Miki, Norihisa. / Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. pp. 2080-2082
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