Microfluidic hydrostatic deposition patterning for a confined hepatocyte-biliary epithelial cell co-culture system

Yuyang Lee, Ryo Sudo, Tomoya Komatsu, Norihisa Miki, Toshihiro Mitaka, Mariko Ikeda, Kazuo Tanishita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Reconstruction of hepato-biliary networks is very important to maintaining liver organoids in vitro, since the accumulation of bile has been proven to be toxic to hepatocytes, especially in long-term culture. We have developed culture methods for reconstructing bile canaliculi (BC) and bile ducts (BDs) formed by small hepatocytes (SHs) which are hepatic progenitor cells, and biliary epithelial cells (BECs), respectively. To study the mechanism of biliary excretion, we need to establish a co-culture system for SHs and BECs. A limitation of conventional culture methods in investigating the mechanisms of hepato-biliary connections is the difficulty to observe the dynamic interactions between SHs and BECs in culture. In addition, patterned constructs do not form in conventional cultures due to the lack of organization of cells. A system that allows more complex dynamics between SHs and BECs to occur is needed in order to investigate the interactions between these cell types. Here, we present an approach called Microfluidic Hydrostatic Deposition Patterning (MHDP) that patterns SH and BEC colonies with defined shape and position, which promotes productive interactions between the cells and eventually induce the formation of heterogeneous tissues integration which is thought to be a prototype of complete biliary networks.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PublisherIEEE Computer Society
Pages10-15
Number of pages6
ISBN (Print)9781457713613
DOIs
Publication statusPublished - 2011 Jan 1
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
Duration: 2011 Nov 62011 Nov 9

Publication series

Name2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CountryJapan
CityNagoya
Period11/11/611/11/9

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

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