Microfluidic paper-based analytical device for fluorescence detection of lactoferrin in tear fluid

K. Yamada, S. Takaki, K. Suzuki, D. Citterio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A microfluidic paper-based analytical device (μPAD) for the determination of lactoferrin in human tear fluid has been developed. By using the fluorescence signal emitted from lactoferrin-terbium complexes, an antibody-free sensing system has been achieved. Application of 2.5 μL of sample onto a μPAD allowed for the detection of lactoferrin concentration in human tear fluid. The selectivity for lactoferrin among the primary constituents of tear fluid has been confirmed. Furthermore, the accuracy of the developed μPAD has been evaluated by comparing the analysis results of real samples with the conventional ELISA method.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages895-897
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

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Keywords

  • Chemical sensor
  • Lactoferrin
  • Microfluidic
  • Paper-based device

ASJC Scopus subject areas

  • Bioengineering

Cite this

Yamada, K., Takaki, S., Suzuki, K., & Citterio, D. (2013). Microfluidic paper-based analytical device for fluorescence detection of lactoferrin in tear fluid. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 895-897). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.