Microfluidic Paper-Based Analytical Devices for Colorimetric Detection of Lactoferrin

Hiroko Kudo, Kento Maejima, Yuki Hiruta, Daniel Citterio

Research output: Contribution to journalArticle

Abstract

Lactoferrin is an abundant glycoprotein in human body fluids and is known as a biomarker for various diseases. Therefore, point-of-care testing (POCT) for lactoferrin is of interest. Microfluidic paper-based analytical devices (µPADs) have gained a lot of attention as next-generation POCT device candidates, due to their inexpensiveness, operational simplicity, and being safely disposable. This work presents a colorimetric sensing approach for quantitative lactoferrin analysis. The detection mechanism takes advantage of the high affinity of lactoferrin to ferric ions (Fe3+). Lactoferrin is able to displace an indicator from a colorimetric 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP)-Fe3+ complex, resulting in a color change. A 5-Br-PADAP-Fe3+ complex was encapsulated into water-dispersible poly(styrene-block-vinylpyrrolidone) particles, whose physical entrapment in the cellulosic fiber network results in the immobilization of the complex to the paper matrix. The complex-encapsulating particles showed a color change response in accordance with lactoferrin concentration. Both color intensity-based paper well plates and distance readout-based µPADs are demonstrated. Color intensity-based devices allowed quantitative analysis of lactoferrin concentrations with a limit of detection of 110 µg/mL, using a smartphone and a color readout app. On the other hand, distance readout-based µPADs showed changes of the length of colored sections in accordance with lactoferrin concentration. In summary, we successfully developed both colorimetric intensity-based paper wells and distance-based µPADs for lactoferrin detection. This work demonstrates a user-friendly colorimetric analysis platform for lactoferrin without requiring lab equipment and expensive antibodies.

Original languageEnglish
JournalSLAS Technology
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Lactoferrin
Microfluidics
Color
Equipment and Supplies
Colorimetric analysis
Glycoproteins
Smartphones
Body fluids
Testing
Biomarkers
Chemical analysis
Application programs
Antibodies
Styrene
Fibers
Ions
Body Fluids
Human Body
Immobilization
Limit of Detection

Keywords

  • colorimetric detection
  • lactoferrin
  • microfluidic paper-based analytical devices
  • µPADs

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

Cite this

Microfluidic Paper-Based Analytical Devices for Colorimetric Detection of Lactoferrin. / Kudo, Hiroko; Maejima, Kento; Hiruta, Yuki; Citterio, Daniel.

In: SLAS Technology, 01.01.2019.

Research output: Contribution to journalArticle

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