Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

S. Chung, R. Sudo, I. Zervantonakis, T. Rimchala, P. J. Mack, C. R. Wan, V. Vickerman, R. D. Kamm

Research output: Contribution to conferencePaper

Abstract

Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

Original languageEnglish
Pages24-26
Number of pages3
Publication statusPublished - 2008 Jan 1
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Capillary morphogenesis
  • Cell migration
  • Microfluidic

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Chung, S., Sudo, R., Zervantonakis, I., Rimchala, T., Mack, P. J., Wan, C. R., Vickerman, V., & Kamm, R. D. (2008). Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. 24-26. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.