Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

S. Chung, Ryo Sudo, I. Zervantonakis, T. Rimchala, P. J. Mack, C. R. Wan, V. Vickerman, R. D. Kamm

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages24-26
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Fingerprint

Microfluidics
Tumors
Monolayers
Cells
Chemotactic Factors
Endothelial cells
Imaging techniques
Experiments

Keywords

  • Capillary morphogenesis
  • Cell migration
  • Microfluidic

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chung, S., Sudo, R., Zervantonakis, I., Rimchala, T., Mack, P. J., Wan, C. R., ... Kamm, R. D. (2008). Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 24-26). Chemical and Biological Microsystems Society.

Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. / Chung, S.; Sudo, Ryo; Zervantonakis, I.; Rimchala, T.; Mack, P. J.; Wan, C. R.; Vickerman, V.; Kamm, R. D.

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 24-26.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chung, S, Sudo, R, Zervantonakis, I, Rimchala, T, Mack, PJ, Wan, CR, Vickerman, V & Kamm, RD 2008, Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 24-26, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08/10/12.
Chung S, Sudo R, Zervantonakis I, Rimchala T, Mack PJ, Wan CR et al. Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 24-26
Chung, S. ; Sudo, Ryo ; Zervantonakis, I. ; Rimchala, T. ; Mack, P. J. ; Wan, C. R. ; Vickerman, V. ; Kamm, R. D. / Microfluidic platform to study three dimensional cell migration & capillary morphogenesis. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 24-26
@inproceedings{e71bbb36283b4af1bfef008b16fe1c91,
title = "Microfluidic platform to study three dimensional cell migration & capillary morphogenesis",
abstract = "Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.",
keywords = "Capillary morphogenesis, Cell migration, Microfluidic",
author = "S. Chung and Ryo Sudo and I. Zervantonakis and T. Rimchala and Mack, {P. J.} and Wan, {C. R.} and V. Vickerman and Kamm, {R. D.}",
year = "2008",
language = "English",
pages = "24--26",
booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Microfluidic platform to study three dimensional cell migration & capillary morphogenesis

AU - Chung, S.

AU - Sudo, Ryo

AU - Zervantonakis, I.

AU - Rimchala, T.

AU - Mack, P. J.

AU - Wan, C. R.

AU - Vickerman, V.

AU - Kamm, R. D.

PY - 2008

Y1 - 2008

N2 - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

AB - Here we present a new microfluidic platform that allows for the simultaneous imaging of cells migrating through a 3D matrix, production of a stable, concentration gradient of chemoattractant, and side-by-side comparisons of control and test conditions to normalize for sample-to-sample variability. We demonstrate the system here in a study of the migration of two tumor cell lines and the sprouting of endothelial cells into the matrix from a monolayer. We also show results using the same system to investigate the transmigration of a tumor cell across an endothelial monolayer. These experiments demonstrate the wide range of capabilities of the new system.

KW - Capillary morphogenesis

KW - Cell migration

KW - Microfluidic

UR - http://www.scopus.com/inward/record.url?scp=84902478658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902478658&partnerID=8YFLogxK

M3 - Conference contribution

SP - 24

EP - 26

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

PB - Chemical and Biological Microsystems Society

ER -