TY - JOUR
T1 - Microglena redcarensis sp. nov. (Volvocales, Chlorophyceae), a brackish water chlamydomonad with contractile vacuoles
AU - Nakada, Takashi
AU - Takahashi, Shunsuke
AU - Tomita, Masaru
N1 - Funding Information:
We are grateful to Mr. Satoshi Ikuta (student at Tsuruoka Chuo High School and Research Assistants at the Institute for Advanced Biosciences, Keio University) for technical support. This work was supported by grants from the Institute for Fermentation, Osaka (to TN), and Yamagata Prefectural Government and Tsuruoka City (to MT).
Publisher Copyright:
© 2018 Japanese Society of Phycology
PY - 2018/10
Y1 - 2018/10
N2 - In 1960, Butcher isolated a strain of unicellular green alga, ‘Chlamydomonas subehrenbergii’ SAG 18.89, from a brackish water locality in England, facing the North Sea. Although C. subehrenbergii Butcher was previously described by the same author in 1959, the strain SAG 18.89 is in fact morphologically different, possessing shorter flagella, a different-shaped papilla, and different-sized vegetative cells. Light and fluorescence microscopy, molecular phylogenetic analyses, and ITS2 comparisons revealed that this strain represents an undescribed species of the genus Microglena (Volvocales, Chlorophyceae), described here as M. redcarensis sp. nov. The presence of contractile vacuoles, which are osmoregulatory organelles, suggests potential inhabitation in freshwater. Contractile vacuole activity and growth of M. redcarensis were therefore examined in media of different salinity.
AB - In 1960, Butcher isolated a strain of unicellular green alga, ‘Chlamydomonas subehrenbergii’ SAG 18.89, from a brackish water locality in England, facing the North Sea. Although C. subehrenbergii Butcher was previously described by the same author in 1959, the strain SAG 18.89 is in fact morphologically different, possessing shorter flagella, a different-shaped papilla, and different-sized vegetative cells. Light and fluorescence microscopy, molecular phylogenetic analyses, and ITS2 comparisons revealed that this strain represents an undescribed species of the genus Microglena (Volvocales, Chlorophyceae), described here as M. redcarensis sp. nov. The presence of contractile vacuoles, which are osmoregulatory organelles, suggests potential inhabitation in freshwater. Contractile vacuole activity and growth of M. redcarensis were therefore examined in media of different salinity.
KW - 18S rRNA
KW - ITS2 rDNA
KW - fluorescence microscopy
KW - mitochondria
KW - molecular phylogeny
KW - new species
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U2 - 10.1111/pre.12330
DO - 10.1111/pre.12330
M3 - Article
AN - SCOPUS:85054083992
VL - 66
SP - 310
EP - 317
JO - Phycological Research
JF - Phycological Research
SN - 1322-0829
IS - 4
ER -