Abstract
In 1960, Butcher isolated a strain of unicellular green alga, ‘Chlamydomonas subehrenbergii’ SAG 18.89, from a brackish water locality in England, facing the North Sea. Although C. subehrenbergii Butcher was previously described by the same author in 1959, the strain SAG 18.89 is in fact morphologically different, possessing shorter flagella, a different-shaped papilla, and different-sized vegetative cells. Light and fluorescence microscopy, molecular phylogenetic analyses, and ITS2 comparisons revealed that this strain represents an undescribed species of the genus Microglena (Volvocales, Chlorophyceae), described here as M. redcarensis sp. nov. The presence of contractile vacuoles, which are osmoregulatory organelles, suggests potential inhabitation in freshwater. Contractile vacuole activity and growth of M. redcarensis were therefore examined in media of different salinity.
| Original language | English |
|---|---|
| Pages (from-to) | 310-317 |
| Number of pages | 8 |
| Journal | Phycological Research |
| Volume | 66 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2018 Oct 1 |
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Keywords
- 18S rRNA
- fluorescence microscopy
- ITS2 rDNA
- mitochondria
- molecular phylogeny
- new species
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Physiology
- Aquatic Science
- Agricultural and Biological Sciences (miscellaneous)
- Plant Science
Cite this
Microglena redcarensis sp. nov. (Volvocales, Chlorophyceae), a brackish water chlamydomonad with contractile vacuoles. / Nakada, Takashi; Takahashi, Shunsuke; Tomita, Masaru.
In: Phycological Research, Vol. 66, No. 4, 01.10.2018, p. 310-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microglena redcarensis sp. nov. (Volvocales, Chlorophyceae), a brackish water chlamydomonad with contractile vacuoles
AU - Nakada, Takashi
AU - Takahashi, Shunsuke
AU - Tomita, Masaru
PY - 2018/10/1
Y1 - 2018/10/1
N2 - In 1960, Butcher isolated a strain of unicellular green alga, ‘Chlamydomonas subehrenbergii’ SAG 18.89, from a brackish water locality in England, facing the North Sea. Although C. subehrenbergii Butcher was previously described by the same author in 1959, the strain SAG 18.89 is in fact morphologically different, possessing shorter flagella, a different-shaped papilla, and different-sized vegetative cells. Light and fluorescence microscopy, molecular phylogenetic analyses, and ITS2 comparisons revealed that this strain represents an undescribed species of the genus Microglena (Volvocales, Chlorophyceae), described here as M. redcarensis sp. nov. The presence of contractile vacuoles, which are osmoregulatory organelles, suggests potential inhabitation in freshwater. Contractile vacuole activity and growth of M. redcarensis were therefore examined in media of different salinity.
AB - In 1960, Butcher isolated a strain of unicellular green alga, ‘Chlamydomonas subehrenbergii’ SAG 18.89, from a brackish water locality in England, facing the North Sea. Although C. subehrenbergii Butcher was previously described by the same author in 1959, the strain SAG 18.89 is in fact morphologically different, possessing shorter flagella, a different-shaped papilla, and different-sized vegetative cells. Light and fluorescence microscopy, molecular phylogenetic analyses, and ITS2 comparisons revealed that this strain represents an undescribed species of the genus Microglena (Volvocales, Chlorophyceae), described here as M. redcarensis sp. nov. The presence of contractile vacuoles, which are osmoregulatory organelles, suggests potential inhabitation in freshwater. Contractile vacuole activity and growth of M. redcarensis were therefore examined in media of different salinity.
KW - 18S rRNA
KW - fluorescence microscopy
KW - ITS2 rDNA
KW - mitochondria
KW - molecular phylogeny
KW - new species
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UR - http://www.scopus.com/inward/citedby.url?scp=85054083992&partnerID=8YFLogxK
U2 - 10.1111/pre.12330
DO - 10.1111/pre.12330
M3 - Article
AN - SCOPUS:85054083992
VL - 66
SP - 310
EP - 317
JO - Phycological Research
JF - Phycological Research
SN - 1322-0829
IS - 4
ER -