Microglia-specific localisation of a novel calcium binding protein, Iba1

Daisuke Ito, Yoshinori Imai, Keiko Ohsawa, Kazuyuki Nakajima, Yasuo Fukuuchi, Shinichi Kohsaka

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Abstract

Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain: respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo. and that Tba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalMolecular Brain Research
Volume57
Issue number1
DOIs
Publication statusPublished - 1998 Jun 1
Externally publishedYes

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Keywords

  • Calcium binding protein
  • Facial nerve axotomy
  • Iba1
  • Immunocytochemistry
  • Immunohistochemistry
  • Microglia

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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