Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation

Kenichi Nagase, Risa Shukuwa, Takahiro Onuma, Masayuki Yamato, Naoya Takeda, Teruo Okano

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

There is a great demand for effective cell separation techniques that do not require the labeling of cell surfaces for applications in cell transplantation therapy and cell analysis. In the present study, we prepared thermoresponsive convex or concave substrates with circular hole, cylindrical pillar, and line patterns of various sizes as thermally modulated cell separation materials through the combination of thermal nano-imprinted lithography and subsequent surface-initiated atom transfer radical polymerization of poly(N-isopropylacrylamide). Three types of human cells, human umbilical vein endothelial cells, normal human dermal fibroblasts, and human skeletal muscle myoblast cells, which are commonly used in cardiovascular tissue engineering, were employed in this study. All three cell types could adhere to the prepared thermoresponsive micro- or nano-imprinted substrates at 37 °C and detached at 20 °C. The specific cell adhesion and detachment properties were different for each cell type, and they could be altered simply by changing the pattern shapes and sizes of the surface. In particular, large differences between the three cell types were obtained on the 2 μm hole pattern. Using this difference in cell adhesion properties, thermally modulated cell separation application was achieved by successively incubating at 37 °C and 20 °C. Thus, our thermoresponsive micro/nano-imprinted substrates can be utilized as cooperative cell separating materials by combining appropriate convex or concave patterns and mild temperature changes.

Original languageEnglish
Pages (from-to)5924-5930
Number of pages7
JournalJournal of Materials Chemistry B
Volume5
Issue number30
DOIs
Publication statusPublished - 2017

Fingerprint

Cell Separation
Polymers
Cell adhesion
Substrates
Atom transfer radical polymerization
Endothelial cells
Fibroblasts
Tissue engineering
Labeling
Lithography
Muscle
Cell Adhesion
Cells
Skeletal Myoblasts
Cell Transplantation
Human Umbilical Vein Endothelial Cells
Tissue Engineering
Cell- and Tissue-Based Therapy
Polymerization
Muscle Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Medicine(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation. / Nagase, Kenichi; Shukuwa, Risa; Onuma, Takahiro; Yamato, Masayuki; Takeda, Naoya; Okano, Teruo.

In: Journal of Materials Chemistry B, Vol. 5, No. 30, 2017, p. 5924-5930.

Research output: Contribution to journalArticle

Nagase, Kenichi ; Shukuwa, Risa ; Onuma, Takahiro ; Yamato, Masayuki ; Takeda, Naoya ; Okano, Teruo. / Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation. In: Journal of Materials Chemistry B. 2017 ; Vol. 5, No. 30. pp. 5924-5930.
@article{786023f0e514406cad3c0e9eefb49530,
title = "Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation",
abstract = "There is a great demand for effective cell separation techniques that do not require the labeling of cell surfaces for applications in cell transplantation therapy and cell analysis. In the present study, we prepared thermoresponsive convex or concave substrates with circular hole, cylindrical pillar, and line patterns of various sizes as thermally modulated cell separation materials through the combination of thermal nano-imprinted lithography and subsequent surface-initiated atom transfer radical polymerization of poly(N-isopropylacrylamide). Three types of human cells, human umbilical vein endothelial cells, normal human dermal fibroblasts, and human skeletal muscle myoblast cells, which are commonly used in cardiovascular tissue engineering, were employed in this study. All three cell types could adhere to the prepared thermoresponsive micro- or nano-imprinted substrates at 37 °C and detached at 20 °C. The specific cell adhesion and detachment properties were different for each cell type, and they could be altered simply by changing the pattern shapes and sizes of the surface. In particular, large differences between the three cell types were obtained on the 2 μm hole pattern. Using this difference in cell adhesion properties, thermally modulated cell separation application was achieved by successively incubating at 37 °C and 20 °C. Thus, our thermoresponsive micro/nano-imprinted substrates can be utilized as cooperative cell separating materials by combining appropriate convex or concave patterns and mild temperature changes.",
author = "Kenichi Nagase and Risa Shukuwa and Takahiro Onuma and Masayuki Yamato and Naoya Takeda and Teruo Okano",
year = "2017",
doi = "10.1039/c7tb01251a",
language = "English",
volume = "5",
pages = "5924--5930",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "30",

}

TY - JOUR

T1 - Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation

AU - Nagase, Kenichi

AU - Shukuwa, Risa

AU - Onuma, Takahiro

AU - Yamato, Masayuki

AU - Takeda, Naoya

AU - Okano, Teruo

PY - 2017

Y1 - 2017

N2 - There is a great demand for effective cell separation techniques that do not require the labeling of cell surfaces for applications in cell transplantation therapy and cell analysis. In the present study, we prepared thermoresponsive convex or concave substrates with circular hole, cylindrical pillar, and line patterns of various sizes as thermally modulated cell separation materials through the combination of thermal nano-imprinted lithography and subsequent surface-initiated atom transfer radical polymerization of poly(N-isopropylacrylamide). Three types of human cells, human umbilical vein endothelial cells, normal human dermal fibroblasts, and human skeletal muscle myoblast cells, which are commonly used in cardiovascular tissue engineering, were employed in this study. All three cell types could adhere to the prepared thermoresponsive micro- or nano-imprinted substrates at 37 °C and detached at 20 °C. The specific cell adhesion and detachment properties were different for each cell type, and they could be altered simply by changing the pattern shapes and sizes of the surface. In particular, large differences between the three cell types were obtained on the 2 μm hole pattern. Using this difference in cell adhesion properties, thermally modulated cell separation application was achieved by successively incubating at 37 °C and 20 °C. Thus, our thermoresponsive micro/nano-imprinted substrates can be utilized as cooperative cell separating materials by combining appropriate convex or concave patterns and mild temperature changes.

AB - There is a great demand for effective cell separation techniques that do not require the labeling of cell surfaces for applications in cell transplantation therapy and cell analysis. In the present study, we prepared thermoresponsive convex or concave substrates with circular hole, cylindrical pillar, and line patterns of various sizes as thermally modulated cell separation materials through the combination of thermal nano-imprinted lithography and subsequent surface-initiated atom transfer radical polymerization of poly(N-isopropylacrylamide). Three types of human cells, human umbilical vein endothelial cells, normal human dermal fibroblasts, and human skeletal muscle myoblast cells, which are commonly used in cardiovascular tissue engineering, were employed in this study. All three cell types could adhere to the prepared thermoresponsive micro- or nano-imprinted substrates at 37 °C and detached at 20 °C. The specific cell adhesion and detachment properties were different for each cell type, and they could be altered simply by changing the pattern shapes and sizes of the surface. In particular, large differences between the three cell types were obtained on the 2 μm hole pattern. Using this difference in cell adhesion properties, thermally modulated cell separation application was achieved by successively incubating at 37 °C and 20 °C. Thus, our thermoresponsive micro/nano-imprinted substrates can be utilized as cooperative cell separating materials by combining appropriate convex or concave patterns and mild temperature changes.

UR - http://www.scopus.com/inward/record.url?scp=85026831796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026831796&partnerID=8YFLogxK

U2 - 10.1039/c7tb01251a

DO - 10.1039/c7tb01251a

M3 - Article

AN - SCOPUS:85026831796

VL - 5

SP - 5924

EP - 5930

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 30

ER -