Abstract
Based on our concept of living single-cell protein analysis where single cell is processed in microchannel and secreted proteins are analyzed in nanochannel, we developed a micro/nano-integrated fluidic device where micro/nanochannels are hierarchically structured. All the 11 unit operations of single-cell analysis including single-cell isolation, femtoliter sampling, antigen-antibody reaction are integrated in femtoliter-to-picoliter space, and the working principle was verified by detecting a signal from 11 fL volume of 75 nM standard C-reactive protein solution (~500 molecules). This device can contribute to medicine and biology by providing a novel tool for elucidation of single-cell-level mechanism of cancer or immune diseases.
| Original language | English |
|---|---|
| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 132-134 |
| Number of pages | 3 |
| ISBN (Electronic) | 9781510897571 |
| Publication status | Published - 2018 Jan 1 |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 2018 Nov 11 → 2018 Nov 15 |
Publication series
| Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Volume | 1 |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Country | Taiwan, Province of China |
| City | Kaohsiung |
| Period | 18/11/11 → 18/11/15 |
Keywords
- ELISA
- Extended-nano space
- Microfluidics
- Nanofluidics
- Single cell
ASJC Scopus subject areas
- Chemistry(all)
- Bioengineering
- Chemical Engineering (miscellaneous)
- Control and Systems Engineering
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Cite this
Nakao, T., Kazoe, Y., Morikawa, K., Yoshizaki, A., Mawatari, K., & Kitamori, T. (2018). Micro/nano-integrated fluidic device for living single-cell protein analysis. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 132-134). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 1). Chemical and Biological Microsystems Society.