@inproceedings{b8bf0a911d7d494aad1aba9d220a1074,
title = "Micro/nano-integrated fluidic device for living single-cell protein analysis",
abstract = "Based on our concept of living single-cell protein analysis where single cell is processed in microchannel and secreted proteins are analyzed in nanochannel, we developed a micro/nano-integrated fluidic device where micro/nanochannels are hierarchically structured. All the 11 unit operations of single-cell analysis including single-cell isolation, femtoliter sampling, antigen-antibody reaction are integrated in femtoliter-to-picoliter space, and the working principle was verified by detecting a signal from 11 fL volume of 75 nM standard C-reactive protein solution (~500 molecules). This device can contribute to medicine and biology by providing a novel tool for elucidation of single-cell-level mechanism of cancer or immune diseases.",
keywords = "ELISA, Extended-nano space, Microfluidics, Nanofluidics, Single cell",
author = "Tatsuro Nakao and Yutaka Kazoe and Kyojiro Morikawa and Ayumi Yoshizaki and Kazuma Mawatari and Takehiko Kitamori",
note = "Funding Information: This study was supported by JST CREST JPMJCR14G1 and JSPS KAKENHI JP17J10031. Publisher Copyright: Copyright{\textcopyright} (2018) by Chemical and Biological Microsystems Society.All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",
year = "2018",
language = "English",
series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
publisher = "Chemical and Biological Microsystems Society",
pages = "132--134",
booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",
}
