MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration

Zhenfeng Duan, Edwin Choy, David Harmon, Xianzhe Liu, Michiro Susa, Henry Mankin, Francis Hornicek

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178 Citations (Scopus)

Abstract

microRNAs (miRNA,miR) play an important role in cancer cell growth andmigration;however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in theG1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p mayplay a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's functionmay provide therapeutic benefits in osteosarcoma.

Original languageEnglish
Pages (from-to)1337-1345
Number of pages9
JournalMolecular cancer therapeutics
Volume10
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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