MicroRNAs in neural stem cells and neurogenesis

Hironori Kawahara, Takao Imai, Hideyuki Okano

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

MicroRNA (miRNA) is a type of short-length (~22 nt) non-coding RNA. Most miRNAs are transcribed by RNA polymerase II and processed by Drosha-DGCR8 and Dicer complexes in the cropping and dicing steps, respectively. miRNAs are exported by exportin-5 from the nucleus to the cytoplasm after cropping. Trimmed mature miRNA is loaded and targets mRNA at the 3' or 5' untranslated region (UTR) by recognition of base-pairing in the miRNA-loaded RISC, where it is involved in gene silencing including translational repression and/or degradation along with deadenylation. Recent studies have shown that miRNA participates in various biological functions including cell fate decision, developmental timing regulation, apoptosis, and tumorigenesis. Analyses of miRNA expression profiles have demonstrated tissue- and stage-specific miRNAs including the let-7 family, miR-124, and miR-9, which regulate the differentiation of embryonic stem cells and/or neurogenesis. This review focuses on RNA-binding protein-mediated miRNA biogenesis during neurogenesis. These miRNA biogenesis-relating proteins have also been linked to human diseases because their mutations can cause several nervous system disorders. Moreover, defects in core proteins involved in miRNA biogenesis including Drosha, DGCR8, and Dicer promote tumorigenesis. Thus, the study of not only mature miRNA function but also miRNA biogenesis steps is likely to be important.

Original languageEnglish
Article numberArticle 30
Pages (from-to)1-13
Number of pages13
JournalFrontiers in Neuroscience
Issue numberMAR
DOIs
Publication statusPublished - 2012

Fingerprint

Neural Stem Cells
Neurogenesis
MicroRNAs
Carcinogenesis
Karyopherins
Untranslated RNA
RNA-Binding Proteins
RNA Polymerase II
5' Untranslated Regions
Gene Silencing
Embryonic Stem Cells
Nervous System Diseases
Base Pairing
Cytoplasm
Proteins

Keywords

  • Let-7
  • Lin28
  • Microrna biogenesis
  • Musashi1
  • Neural progenitor cells
  • Neural stem cells
  • Neurogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

MicroRNAs in neural stem cells and neurogenesis. / Kawahara, Hironori; Imai, Takao; Okano, Hideyuki.

In: Frontiers in Neuroscience, No. MAR, Article 30, 2012, p. 1-13.

Research output: Contribution to journalArticle

Kawahara, Hironori ; Imai, Takao ; Okano, Hideyuki. / MicroRNAs in neural stem cells and neurogenesis. In: Frontiers in Neuroscience. 2012 ; No. MAR. pp. 1-13.
@article{3d1c92e2e6e341e29a4460c9c58be9c0,
title = "MicroRNAs in neural stem cells and neurogenesis",
abstract = "MicroRNA (miRNA) is a type of short-length (~22 nt) non-coding RNA. Most miRNAs are transcribed by RNA polymerase II and processed by Drosha-DGCR8 and Dicer complexes in the cropping and dicing steps, respectively. miRNAs are exported by exportin-5 from the nucleus to the cytoplasm after cropping. Trimmed mature miRNA is loaded and targets mRNA at the 3' or 5' untranslated region (UTR) by recognition of base-pairing in the miRNA-loaded RISC, where it is involved in gene silencing including translational repression and/or degradation along with deadenylation. Recent studies have shown that miRNA participates in various biological functions including cell fate decision, developmental timing regulation, apoptosis, and tumorigenesis. Analyses of miRNA expression profiles have demonstrated tissue- and stage-specific miRNAs including the let-7 family, miR-124, and miR-9, which regulate the differentiation of embryonic stem cells and/or neurogenesis. This review focuses on RNA-binding protein-mediated miRNA biogenesis during neurogenesis. These miRNA biogenesis-relating proteins have also been linked to human diseases because their mutations can cause several nervous system disorders. Moreover, defects in core proteins involved in miRNA biogenesis including Drosha, DGCR8, and Dicer promote tumorigenesis. Thus, the study of not only mature miRNA function but also miRNA biogenesis steps is likely to be important.",
keywords = "Let-7, Lin28, Microrna biogenesis, Musashi1, Neural progenitor cells, Neural stem cells, Neurogenesis",
author = "Hironori Kawahara and Takao Imai and Hideyuki Okano",
year = "2012",
doi = "10.3389/fnins.2012.00030",
language = "English",
pages = "1--13",
journal = "Frontiers in Neuroscience",
issn = "1662-4548",
publisher = "Frontiers Research Foundation",
number = "MAR",

}

TY - JOUR

T1 - MicroRNAs in neural stem cells and neurogenesis

AU - Kawahara, Hironori

AU - Imai, Takao

AU - Okano, Hideyuki

PY - 2012

Y1 - 2012

N2 - MicroRNA (miRNA) is a type of short-length (~22 nt) non-coding RNA. Most miRNAs are transcribed by RNA polymerase II and processed by Drosha-DGCR8 and Dicer complexes in the cropping and dicing steps, respectively. miRNAs are exported by exportin-5 from the nucleus to the cytoplasm after cropping. Trimmed mature miRNA is loaded and targets mRNA at the 3' or 5' untranslated region (UTR) by recognition of base-pairing in the miRNA-loaded RISC, where it is involved in gene silencing including translational repression and/or degradation along with deadenylation. Recent studies have shown that miRNA participates in various biological functions including cell fate decision, developmental timing regulation, apoptosis, and tumorigenesis. Analyses of miRNA expression profiles have demonstrated tissue- and stage-specific miRNAs including the let-7 family, miR-124, and miR-9, which regulate the differentiation of embryonic stem cells and/or neurogenesis. This review focuses on RNA-binding protein-mediated miRNA biogenesis during neurogenesis. These miRNA biogenesis-relating proteins have also been linked to human diseases because their mutations can cause several nervous system disorders. Moreover, defects in core proteins involved in miRNA biogenesis including Drosha, DGCR8, and Dicer promote tumorigenesis. Thus, the study of not only mature miRNA function but also miRNA biogenesis steps is likely to be important.

AB - MicroRNA (miRNA) is a type of short-length (~22 nt) non-coding RNA. Most miRNAs are transcribed by RNA polymerase II and processed by Drosha-DGCR8 and Dicer complexes in the cropping and dicing steps, respectively. miRNAs are exported by exportin-5 from the nucleus to the cytoplasm after cropping. Trimmed mature miRNA is loaded and targets mRNA at the 3' or 5' untranslated region (UTR) by recognition of base-pairing in the miRNA-loaded RISC, where it is involved in gene silencing including translational repression and/or degradation along with deadenylation. Recent studies have shown that miRNA participates in various biological functions including cell fate decision, developmental timing regulation, apoptosis, and tumorigenesis. Analyses of miRNA expression profiles have demonstrated tissue- and stage-specific miRNAs including the let-7 family, miR-124, and miR-9, which regulate the differentiation of embryonic stem cells and/or neurogenesis. This review focuses on RNA-binding protein-mediated miRNA biogenesis during neurogenesis. These miRNA biogenesis-relating proteins have also been linked to human diseases because their mutations can cause several nervous system disorders. Moreover, defects in core proteins involved in miRNA biogenesis including Drosha, DGCR8, and Dicer promote tumorigenesis. Thus, the study of not only mature miRNA function but also miRNA biogenesis steps is likely to be important.

KW - Let-7

KW - Lin28

KW - Microrna biogenesis

KW - Musashi1

KW - Neural progenitor cells

KW - Neural stem cells

KW - Neurogenesis

UR - http://www.scopus.com/inward/record.url?scp=84862206162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862206162&partnerID=8YFLogxK

U2 - 10.3389/fnins.2012.00030

DO - 10.3389/fnins.2012.00030

M3 - Article

SP - 1

EP - 13

JO - Frontiers in Neuroscience

JF - Frontiers in Neuroscience

SN - 1662-4548

IS - MAR

M1 - Article 30

ER -