Microsatellite analysis of chromosome 10q in malignant glioma

Hideo Nakamura; Mitsuvoshi Nakao; Hideyuki Saya; Jun Ichi Kuratsu; Yukitaka Ushio

Microsatellite analysis of chromosome 10q in malignant glioma

Hideo Nakamura, Mitsuvoshi Nakao, Hideyuki Saya, Jun Ichi Kuratsu, Yukitaka Ushio

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Abstract

Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.

Original language	English
Number of pages	1
Journal	Japanese Journal of Human Genetics
Volume	41
Issue number	1
Publication status	Published - 1996 Dec 1
Externally published	Yes

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Genetics(clinical)

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Nakamura, H., Nakao, M., Saya, H., Kuratsu, J. I., & Ushio, Y. (1996). Microsatellite analysis of chromosome 10q in malignant glioma. Japanese Journal of Human Genetics, 41(1).

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title = "Microsatellite analysis of chromosome 10q in malignant glioma",

abstract = "Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.",

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AU - Nakamura, Hideo

AU - Nakao, Mitsuvoshi

AU - Saya, Hideyuki

AU - Kuratsu, Jun Ichi

AU - Ushio, Yukitaka

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.

AB - Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.

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Microsatellite analysis of chromosome 10q in malignant glioma

Abstract

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