Abstract
Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.
| Original language | English |
|---|---|
| Pages (from-to) | 57 |
| Number of pages | 1 |
| Journal | Japanese Journal of Human Genetics |
| Volume | 41 |
| Issue number | 1 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics(clinical)
- Genetics
Cite this
Microsatellite analysis of chromosome 10q in malignant glioma. / Nakamura, Hideo; Nakao, Mitsuvoshi; Saya, Hideyuki; Kuratsu, Jun Ichi; Ushio, Yukitaka.
In: Japanese Journal of Human Genetics, Vol. 41, No. 1, 1996, p. 57.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microsatellite analysis of chromosome 10q in malignant glioma
AU - Nakamura, Hideo
AU - Nakao, Mitsuvoshi
AU - Saya, Hideyuki
AU - Kuratsu, Jun Ichi
AU - Ushio, Yukitaka
PY - 1996
Y1 - 1996
N2 - Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.
AB - Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.
UR - http://www.scopus.com/inward/record.url?scp=33748193813&partnerID=8YFLogxK
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M3 - Article
VL - 41
SP - 57
JO - Journal of Human Genetics
JF - Journal of Human Genetics
SN - 1434-5161
IS - 1
ER -