Microsatellite analysis of chromosome 10q in malignant glioma

Hideo Nakamura, Mitsuvoshi Nakao, Hideyuki Saya, Jun Ichi Kuratsu, Yukitaka Ushio

Research output: Contribution to journalArticle

Abstract

Chromosome 10 is frequently deleted in malignant gliomas and the frequency of the deletion is known to be related to clinical and pathological malignancy of the tumor. Therefore, we expect that certain genes which is implicated in malignancy of glioma are located on chromosome 10. Here, we analyze the loss of heterozygosity (LOH) of microsatellite markers on chromosome lOq by PCR using fluorescentry-labelled primer. D10S201, D10S200, D10S603, D10S192, D10S205, D10S222, D10S540, D10S566, D10S530, D10S597, D10S545, D10S587, D10S186, and D10S212 were used for this analysis. LOH of the markers were quantitatively analyzed by an automated DNA fragment analyzer and a software. Using this system, we found LOH in 19 of 43 cases of malignant gliomas, and the most frequent deletion was identified at D10S 566 region.

Original languageEnglish
Number of pages1
JournalJapanese Journal of Human Genetics
Volume41
Issue number1
Publication statusPublished - 1996 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics(clinical)

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  • Cite this

    Nakamura, H., Nakao, M., Saya, H., Kuratsu, J. I., & Ushio, Y. (1996). Microsatellite analysis of chromosome 10q in malignant glioma. Japanese Journal of Human Genetics, 41(1).