Microsatellite markers developed using a next-generation sequencing technique for Neotrogla spp. (Psocodea: Prionoglarididae), cave dwelling insects with sex-reversed genitalia

Yoshitaka Kamimura, Jun Abe, Rodrigo L. Ferreira, Kazunori Yoshizawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The genus Neotrogla (Psocodea: Prinoglarididae) comprises four named species from Brazil. Females of this cave-dwelling insect are characterized by a conspicuous penis-like intromittent organ, termed a gynosome, which is inserted into the vagina-like male genitalia during copulation. Another evolutionarily novel structure, the spermathecal plate, enables a female to simultaneously store two freshly deposited spermatophores (consisting of sperm and possibly nutritious substances) in her sperm storage organ (spermatheca). It is unknown whether the two spermatophores are derived from two different males. To investigate the mating ecology and population genetic structures of these insects with sex-reversed genitalia, 16 novel highly polymorphic microsatellite loci were isolated and characterized based on ~2,275 Mbp genomic sequences from an undescribed Neotrogla species. Our first screening detected 99,888 candidate loci. Similar to other hemipteroid insects studied thus far, AAT motif microsatellites were conspicuously dominant. We further screened 99 sequences, for which 50 pairs of polymerase chain reaction primers were successfully designed. Sixteen of these primers successfully amplified products of the expected size in the 11 Neotrogla sp. individuals collected from two caves. The number of alleles per loci varied from two to nine, with no significant deviation from the Hardy–Weinberg equilibrium in either population. Although the caves sampled were only approximately 1 km apart, significant genetic differentiation was detected between the two populations. In total, 13, 12, 13 and 11 loci were cross-amplified in N. aurora, N. brasiliensis, N. curvata and N. truncata, respectively, indicating the applicability of these microsatellite loci for metapopulation genetic studies in multiple Neotrogla species.

Original languageEnglish
JournalEntomological Science
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Psocodea
caves
genitalia
cave
spermatophore
microsatellite repeats
insect
sperm
insects
loci
gender
spermatophores
copulation
aurora
metapopulation
genetic differentiation
polymerase chain reaction
genetic structure
population genetics
spermatozoa

Keywords

  • cave populations
  • genetic differentiation
  • nuptial gift
  • sex role reversal
  • simple sequence repeat (SSR)

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

@article{e3a04634b3d14786b6e1c5317caf6ca4,
title = "Microsatellite markers developed using a next-generation sequencing technique for Neotrogla spp. (Psocodea: Prionoglarididae), cave dwelling insects with sex-reversed genitalia",
abstract = "The genus Neotrogla (Psocodea: Prinoglarididae) comprises four named species from Brazil. Females of this cave-dwelling insect are characterized by a conspicuous penis-like intromittent organ, termed a gynosome, which is inserted into the vagina-like male genitalia during copulation. Another evolutionarily novel structure, the spermathecal plate, enables a female to simultaneously store two freshly deposited spermatophores (consisting of sperm and possibly nutritious substances) in her sperm storage organ (spermatheca). It is unknown whether the two spermatophores are derived from two different males. To investigate the mating ecology and population genetic structures of these insects with sex-reversed genitalia, 16 novel highly polymorphic microsatellite loci were isolated and characterized based on ~2,275 Mbp genomic sequences from an undescribed Neotrogla species. Our first screening detected 99,888 candidate loci. Similar to other hemipteroid insects studied thus far, AAT motif microsatellites were conspicuously dominant. We further screened 99 sequences, for which 50 pairs of polymerase chain reaction primers were successfully designed. Sixteen of these primers successfully amplified products of the expected size in the 11 Neotrogla sp. individuals collected from two caves. The number of alleles per loci varied from two to nine, with no significant deviation from the Hardy–Weinberg equilibrium in either population. Although the caves sampled were only approximately 1 km apart, significant genetic differentiation was detected between the two populations. In total, 13, 12, 13 and 11 loci were cross-amplified in N. aurora, N. brasiliensis, N. curvata and N. truncata, respectively, indicating the applicability of these microsatellite loci for metapopulation genetic studies in multiple Neotrogla species.",
keywords = "cave populations, genetic differentiation, nuptial gift, sex role reversal, simple sequence repeat (SSR)",
author = "Yoshitaka Kamimura and Jun Abe and Ferreira, {Rodrigo L.} and Kazunori Yoshizawa",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/ens.12339",
language = "English",
journal = "Entomological Science",
issn = "1343-8786",
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T2 - Prionoglarididae), cave dwelling insects with sex-reversed genitalia

AU - Kamimura, Yoshitaka

AU - Abe, Jun

AU - Ferreira, Rodrigo L.

AU - Yoshizawa, Kazunori

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N2 - The genus Neotrogla (Psocodea: Prinoglarididae) comprises four named species from Brazil. Females of this cave-dwelling insect are characterized by a conspicuous penis-like intromittent organ, termed a gynosome, which is inserted into the vagina-like male genitalia during copulation. Another evolutionarily novel structure, the spermathecal plate, enables a female to simultaneously store two freshly deposited spermatophores (consisting of sperm and possibly nutritious substances) in her sperm storage organ (spermatheca). It is unknown whether the two spermatophores are derived from two different males. To investigate the mating ecology and population genetic structures of these insects with sex-reversed genitalia, 16 novel highly polymorphic microsatellite loci were isolated and characterized based on ~2,275 Mbp genomic sequences from an undescribed Neotrogla species. Our first screening detected 99,888 candidate loci. Similar to other hemipteroid insects studied thus far, AAT motif microsatellites were conspicuously dominant. We further screened 99 sequences, for which 50 pairs of polymerase chain reaction primers were successfully designed. Sixteen of these primers successfully amplified products of the expected size in the 11 Neotrogla sp. individuals collected from two caves. The number of alleles per loci varied from two to nine, with no significant deviation from the Hardy–Weinberg equilibrium in either population. Although the caves sampled were only approximately 1 km apart, significant genetic differentiation was detected between the two populations. In total, 13, 12, 13 and 11 loci were cross-amplified in N. aurora, N. brasiliensis, N. curvata and N. truncata, respectively, indicating the applicability of these microsatellite loci for metapopulation genetic studies in multiple Neotrogla species.

AB - The genus Neotrogla (Psocodea: Prinoglarididae) comprises four named species from Brazil. Females of this cave-dwelling insect are characterized by a conspicuous penis-like intromittent organ, termed a gynosome, which is inserted into the vagina-like male genitalia during copulation. Another evolutionarily novel structure, the spermathecal plate, enables a female to simultaneously store two freshly deposited spermatophores (consisting of sperm and possibly nutritious substances) in her sperm storage organ (spermatheca). It is unknown whether the two spermatophores are derived from two different males. To investigate the mating ecology and population genetic structures of these insects with sex-reversed genitalia, 16 novel highly polymorphic microsatellite loci were isolated and characterized based on ~2,275 Mbp genomic sequences from an undescribed Neotrogla species. Our first screening detected 99,888 candidate loci. Similar to other hemipteroid insects studied thus far, AAT motif microsatellites were conspicuously dominant. We further screened 99 sequences, for which 50 pairs of polymerase chain reaction primers were successfully designed. Sixteen of these primers successfully amplified products of the expected size in the 11 Neotrogla sp. individuals collected from two caves. The number of alleles per loci varied from two to nine, with no significant deviation from the Hardy–Weinberg equilibrium in either population. Although the caves sampled were only approximately 1 km apart, significant genetic differentiation was detected between the two populations. In total, 13, 12, 13 and 11 loci were cross-amplified in N. aurora, N. brasiliensis, N. curvata and N. truncata, respectively, indicating the applicability of these microsatellite loci for metapopulation genetic studies in multiple Neotrogla species.

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