Microscopic local measurement of blood flow and oxygen tension in brain microcirculation

Haruyuki Minamitani, Ryota Takahashi, Kousuke Tsukada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A multi-photonic imaging system was proposed for measuring blood flow velocity, vessel diameter and blood oxygen tension pO2 simultaneously with high spatio-temporal resolution in the parenchymatous organ microcirculation, such as pial tissue, by using a closed cranial window and two light sources. FITC-stained erythrocytes was used to visualize the microcirculation, and the fluorescent image was recorded by a high-speed video camera for measuring blood flow velocity. Oxygen tension pO2 was measured by oxygen-dependent quenching of phosphorescent molecules, Pd-TCPP, in the microvessels after irradiation of second harmonic light of Nd:YAG pulse laser (532nm). Animal experiments were performed for investigation of blood flow dynamics and oxygen diffusion phenomenon during acute cerebral ischemia using photochemical thrombus formation in the closed cranial window of male Wistar rats. Experimental results showed specific and significant blood flow and oxygen diffusion phenomena related to the abnormal organ tissues, from those the proposed technique would contribute to the trasnlational research for the clinical medicine, concerned in the ischemic dysfunction, angiogenisis, tumorgenisis and so on.

Original languageEnglish
Pages (from-to)1529-1536+6
JournalIEEJ Transactions on Electronics, Information and Systems
Volume127
Issue number10
DOIs
Publication statusPublished - 2007

Keywords

  • Brain blood flow
  • Fluorescence
  • Microcirculation
  • Oxygen tension
  • Phosphorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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