Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B

Yuki Fujiwara, Katsumi Kasashima, Kuniaki Saito, Miho Fukuda, Akira Fukao, Yumi Sasano, Kunio Inoue, Toshinobu Fujiwara, Hiroshi Sakamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

RNA-binding proteins (RBPs) play a vital role in the post-transcriptional regulation of gene expression during neuronal differentiation and synaptic plasticity. One such RBP family, the neuronal Hu protein family, serves as an early marker of neuronal differentiation and targets several mRNAs containing adenine/uridine-rich elements. Recently, we reported that one of the neuronal Hu proteins, HuD stimulates cap-dependent translation through interactions with eIF4A and poly (A) tail. Nevertheless, little is known with respect to how neuronal Hu proteins contribute to the local translation of target mRNAs in neuronal differentiation. Here, we found that neuronal Hu proteins, but not the ubiquitously expressed HuR protein, directly interact with the light chain of microtubule-associated proteins MAP1B (LC1). We also show that HuD simultaneously binds both RNA and LC1 in vitro and that it tightly associates with microtubules in cells in an LC1-dependent manner, raising the possibility that HuD recruits target mRNAs to microtubules. These results uncover the neuronal binding partners for neuron-specific Hu proteins and suggest the involvement of Hu proteins in microtubule-mediated regulation of mRNA expression within neuronal processes.

Original languageEnglish
Pages (from-to)817-822
Number of pages6
JournalBiochimie
Volume93
Issue number5
DOIs
Publication statusPublished - 2011 May

Fingerprint

RNA-Binding Proteins
Microtubules
Light
Messenger RNA
Neuronal Plasticity
Proteins
Microtubule Proteins
Microtubule-Associated Proteins
Uridine
Differentiation Antigens
Gene Expression Regulation
Protein Biosynthesis
Adenine
Gene expression
Neurons
Plasticity
RNA
microtubule-associated protein 1B

Keywords

  • Hu proteins
  • Microtubule-associated protein
  • Neuronal differentiation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fujiwara, Y., Kasashima, K., Saito, K., Fukuda, M., Fukao, A., Sasano, Y., ... Sakamoto, H. (2011). Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B. Biochimie, 93(5), 817-822. https://doi.org/10.1016/j.biochi.2011.01.008

Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B. / Fujiwara, Yuki; Kasashima, Katsumi; Saito, Kuniaki; Fukuda, Miho; Fukao, Akira; Sasano, Yumi; Inoue, Kunio; Fujiwara, Toshinobu; Sakamoto, Hiroshi.

In: Biochimie, Vol. 93, No. 5, 05.2011, p. 817-822.

Research output: Contribution to journalArticle

Fujiwara, Y, Kasashima, K, Saito, K, Fukuda, M, Fukao, A, Sasano, Y, Inoue, K, Fujiwara, T & Sakamoto, H 2011, 'Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B', Biochimie, vol. 93, no. 5, pp. 817-822. https://doi.org/10.1016/j.biochi.2011.01.008
Fujiwara, Yuki ; Kasashima, Katsumi ; Saito, Kuniaki ; Fukuda, Miho ; Fukao, Akira ; Sasano, Yumi ; Inoue, Kunio ; Fujiwara, Toshinobu ; Sakamoto, Hiroshi. / Microtubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B. In: Biochimie. 2011 ; Vol. 93, No. 5. pp. 817-822.
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