Microtubule disruption induced by estradiol in estrogen receptor-positive and -negative human breast cancer cell lines

Eriko Yokota, Ken Ichinoseki, Yoshihiro Sato

Research output: Contribution to journalArticle

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Abstract

Effects of estrogens on the cytoplasmic microtubule network were examined by the indirect immunofluorescence method using anti-β-tubulin antibody. Estradiol, a naturally occurring estrogen, decreased the amount of cytoplasmic microtubule fibers during interphase in the human breast cancer cell lines MCF-7 and MDA-MB-231. Since MDA-MB-231 is an estrogen receptor-negative cell line, estradiol-induced microtubule disruption seems to be independent of estradiol binding to receptors. The effective concentration of estradiol required for induction of microtubule disruption in 50% of the cells (EC50) was 81 μM for MCF-7 cells and 82 μM for MDA-MB-231 cells. A synthetic estrogen, diethylstilbestrol, also induced a decrease in microtubule fibers, with an EC50 value of 48 μM for MCF-7 cells and 50 μM for MDA-MB-231 cells. When estrogen-treated and microtubule-depolymerized cells were washed and the medium was replaced with fresh, intracellular microtubule networks reappeared within 3 h. When MCF-7 cells were cultured for 4 days with estradiol (50 μM), cell growth was completely inhibited. However, estrone affected the microtubule network and cell proliferation only slightly. These results suggest that estradiol-induced microtubule disruption is closely related to its inhibitory effect on cell growth.

Original languageEnglish
Pages (from-to)1875-1879
Number of pages5
JournalCarcinogenesis
Volume15
Issue number9
Publication statusPublished - 1994 Sep

Fingerprint

Estrogen Receptor
Estradiol
Microtubules
Breast Cancer
Estrogen Receptors
Cells
Breast Neoplasms
Cell Line
Line
Cell
Estrogen
Cell growth
MCF-7 Cells
Estrogens
Fibers
Cell proliferation
Antibodies
Estradiol Congeners
Human
Cell line

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Microtubule disruption induced by estradiol in estrogen receptor-positive and -negative human breast cancer cell lines. / Yokota, Eriko; Ichinoseki, Ken; Sato, Yoshihiro.

In: Carcinogenesis, Vol. 15, No. 9, 09.1994, p. 1875-1879.

Research output: Contribution to journalArticle

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