Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution

Nanae Hangai-Hoger, Amy G. Tsai, Pedro Cabrales, Makoto Suematsu, Marcos Intaglietta

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

OBJECTIVE: To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms. DESIGN: Prospective study. SETTING: University research laboratory. SUBJECTS: Male Golden Syrian hamsters. INTERVENTIONS: Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline. MEASUREMENTS AND MAIN RESULTS: Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter. CONCLUSIONS: Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions.

Original languageEnglish
Pages (from-to)1123-1132
Number of pages10
JournalCritical Care Medicine
Volume35
Issue number4
DOIs
Publication statusPublished - 2007 Apr

Fingerprint

Carbon Monoxide
Sodium Chloride
Cyclic GMP
Blood Volume
Hemodynamics
Cricetinae
Intravenous Injections
Cardiac Output
Observation
Oxadiazoles
Venules
NG-Nitroarginine Methyl Ester
Mesocricetus
Arterioles
Microcirculation
Immunoenzyme Techniques
Vasodilator Agents
Vasodilation
Arterial Pressure
Erythrocytes

Keywords

  • Carbon monoxide
  • Cardiac output
  • Cyclic guanosine monophosphate
  • Hamster window model
  • Microcirculation
  • Vasodilatation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution. / Hangai-Hoger, Nanae; Tsai, Amy G.; Cabrales, Pedro; Suematsu, Makoto; Intaglietta, Marcos.

In: Critical Care Medicine, Vol. 35, No. 4, 04.2007, p. 1123-1132.

Research output: Contribution to journalArticle

Hangai-Hoger, Nanae ; Tsai, Amy G. ; Cabrales, Pedro ; Suematsu, Makoto ; Intaglietta, Marcos. / Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution. In: Critical Care Medicine. 2007 ; Vol. 35, No. 4. pp. 1123-1132.
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AU - Intaglietta, Marcos

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N2 - OBJECTIVE: To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms. DESIGN: Prospective study. SETTING: University research laboratory. SUBJECTS: Male Golden Syrian hamsters. INTERVENTIONS: Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline. MEASUREMENTS AND MAIN RESULTS: Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter. CONCLUSIONS: Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions.

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