TY - JOUR
T1 - Microwave Irradiation as a Promising Method of Sterilization for Acanthamoeba polyphaga in Cultures
AU - Matsumoto, Yukihiro
AU - Ibrahim, Osama M A
AU - Kojima, Takashi
AU - Wakamatsu, Tais H.
AU - Dogru, Murat
AU - Tsubota, Kazuo
PY - 2016/7/27
Y1 - 2016/7/27
N2 - PURPOSE:: To determine the killing effect of microwave irradiation on Acanthamoeba polyphaga. METHODS:: The trophozoites and cysts of A. polyphaga both in water and on agar were exposed to microwave irradiation with a capacity of 750 W for 0, 1, 3, 5, and 10 minutes, respectively. Furthermore, the trophozoites and cysts of A. polyphaga in water were exposed to microwave irradiation with a capacity of 100, 300, and 500 W for 1 minute, respectively. RESULTS:: The trophozoites and cysts of A. polyphaga on agar were completely killed by 3 minutes of microwave irradiation with a capacity of 750 W. The trophozoites and cysts of A. polyphaga in water were completely killed by microwave irradiation with a capacity of 300 W for 1 minute. CONCLUSIONS:: We demonstrate that microwave treatment is effective in killing A. polyphaga both in water and on agar and may be a helpful modality to prevent Acanthamoeba keratitis.
AB - PURPOSE:: To determine the killing effect of microwave irradiation on Acanthamoeba polyphaga. METHODS:: The trophozoites and cysts of A. polyphaga both in water and on agar were exposed to microwave irradiation with a capacity of 750 W for 0, 1, 3, 5, and 10 minutes, respectively. Furthermore, the trophozoites and cysts of A. polyphaga in water were exposed to microwave irradiation with a capacity of 100, 300, and 500 W for 1 minute, respectively. RESULTS:: The trophozoites and cysts of A. polyphaga on agar were completely killed by 3 minutes of microwave irradiation with a capacity of 750 W. The trophozoites and cysts of A. polyphaga in water were completely killed by microwave irradiation with a capacity of 300 W for 1 minute. CONCLUSIONS:: We demonstrate that microwave treatment is effective in killing A. polyphaga both in water and on agar and may be a helpful modality to prevent Acanthamoeba keratitis.
UR - http://www.scopus.com/inward/record.url?scp=84980018620&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84980018620&partnerID=8YFLogxK
U2 - 10.1097/ICO.0000000000000961
DO - 10.1097/ICO.0000000000000961
M3 - Article
C2 - 27467043
AN - SCOPUS:84980018620
SN - 0277-3740
JO - Cornea
JF - Cornea
ER -