Microwave Irradiation as a Promising Method of Sterilization for Acanthamoeba polyphaga in Cultures

Yukihiro Matsumoto, Osama M A Ibrahim, Takashi Kojima, Tais H. Wakamatsu, Murat Dogru, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

PURPOSE:: To determine the killing effect of microwave irradiation on Acanthamoeba polyphaga. METHODS:: The trophozoites and cysts of A. polyphaga both in water and on agar were exposed to microwave irradiation with a capacity of 750 W for 0, 1, 3, 5, and 10 minutes, respectively. Furthermore, the trophozoites and cysts of A. polyphaga in water were exposed to microwave irradiation with a capacity of 100, 300, and 500 W for 1 minute, respectively. RESULTS:: The trophozoites and cysts of A. polyphaga on agar were completely killed by 3 minutes of microwave irradiation with a capacity of 750 W. The trophozoites and cysts of A. polyphaga in water were completely killed by microwave irradiation with a capacity of 300 W for 1 minute. CONCLUSIONS:: We demonstrate that microwave treatment is effective in killing A. polyphaga both in water and on agar and may be a helpful modality to prevent Acanthamoeba keratitis.

Original languageEnglish
JournalCornea
DOIs
Publication statusAccepted/In press - 2016 Jul 27

Fingerprint

Acanthamoeba
Microwaves
Trophozoites
Cysts
Agar
Water
Acanthamoeba Keratitis

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Microwave Irradiation as a Promising Method of Sterilization for Acanthamoeba polyphaga in Cultures. / Matsumoto, Yukihiro; Ibrahim, Osama M A; Kojima, Takashi; Wakamatsu, Tais H.; Dogru, Murat; Tsubota, Kazuo.

In: Cornea, 27.07.2016.

Research output: Contribution to journalArticle

Matsumoto, Yukihiro ; Ibrahim, Osama M A ; Kojima, Takashi ; Wakamatsu, Tais H. ; Dogru, Murat ; Tsubota, Kazuo. / Microwave Irradiation as a Promising Method of Sterilization for Acanthamoeba polyphaga in Cultures. In: Cornea. 2016.
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AU - Ibrahim, Osama M A

AU - Kojima, Takashi

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AU - Dogru, Murat

AU - Tsubota, Kazuo

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N2 - PURPOSE:: To determine the killing effect of microwave irradiation on Acanthamoeba polyphaga. METHODS:: The trophozoites and cysts of A. polyphaga both in water and on agar were exposed to microwave irradiation with a capacity of 750 W for 0, 1, 3, 5, and 10 minutes, respectively. Furthermore, the trophozoites and cysts of A. polyphaga in water were exposed to microwave irradiation with a capacity of 100, 300, and 500 W for 1 minute, respectively. RESULTS:: The trophozoites and cysts of A. polyphaga on agar were completely killed by 3 minutes of microwave irradiation with a capacity of 750 W. The trophozoites and cysts of A. polyphaga in water were completely killed by microwave irradiation with a capacity of 300 W for 1 minute. CONCLUSIONS:: We demonstrate that microwave treatment is effective in killing A. polyphaga both in water and on agar and may be a helpful modality to prevent Acanthamoeba keratitis.

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