Migration of genetically labeled glioma cells after implantation into murine brain

M. Yamada, K. Shimizu, Y. Miyao, T. Hayakawa, Kazunori Nakajima, K. Nakahira, H. Nakagawa, K. Mikoshiba, K. Ikenaka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Murine RSV-M glioma cells were genetically labeled with a retroviral BAG vector carrying the Escherichia coli β-galactosidase gene. The X-gal- positive stable cell line RSV-M/BAG was obtained by the FDG-FACS method. To examine the behavior of glioma cells in the brain, we homografted RSV-M/BAG cells into the brain of C3H/HeN mice as cell suspensions. Individual grafted glioma cells were easily detected by histochemical staining for B- galactosidase (β-gal). Three days after grafting, the β-gal-positive cells were mainly found in the subependymal zone of the lateral ventricle. In addition, some solitary labeled cells were found at locations distant from the injection sites. On the seventh day after implantation, tumor masses were observed and graft-derived glioma cells were migrating bilaterally along the fibers in the corpus callosum. Other labeled cells extended into the brain parenchyma via the perivascular (Virchow-Robin) spaces. Rapid and extensive migration of individual glioma cells was thus clearly demonstrated by intracerebral transplantation of RSV-M/BAG cells.

Original languageEnglish
Pages (from-to)415-423
Number of pages9
JournalJournal of Neuroscience Research
Volume38
Issue number4
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Glioma
Brain
Galactosidases
Songbirds
Inbred C3H Mouse
Corpus Callosum
Lateral Ventricles
Suspensions
Transplantation
Staining and Labeling
Escherichia coli
Transplants
Cell Line
Injections

Keywords

  • glioma
  • migration
  • retroviral vector
  • transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Migration of genetically labeled glioma cells after implantation into murine brain. / Yamada, M.; Shimizu, K.; Miyao, Y.; Hayakawa, T.; Nakajima, Kazunori; Nakahira, K.; Nakagawa, H.; Mikoshiba, K.; Ikenaka, K.

In: Journal of Neuroscience Research, Vol. 38, No. 4, 1994, p. 415-423.

Research output: Contribution to journalArticle

Yamada, M, Shimizu, K, Miyao, Y, Hayakawa, T, Nakajima, K, Nakahira, K, Nakagawa, H, Mikoshiba, K & Ikenaka, K 1994, 'Migration of genetically labeled glioma cells after implantation into murine brain', Journal of Neuroscience Research, vol. 38, no. 4, pp. 415-423. https://doi.org/10.1002/jnr.490380407
Yamada, M. ; Shimizu, K. ; Miyao, Y. ; Hayakawa, T. ; Nakajima, Kazunori ; Nakahira, K. ; Nakagawa, H. ; Mikoshiba, K. ; Ikenaka, K. / Migration of genetically labeled glioma cells after implantation into murine brain. In: Journal of Neuroscience Research. 1994 ; Vol. 38, No. 4. pp. 415-423.
@article{0d0029cd4c584be2b9012f58b6f18c9e,
title = "Migration of genetically labeled glioma cells after implantation into murine brain",
abstract = "Murine RSV-M glioma cells were genetically labeled with a retroviral BAG vector carrying the Escherichia coli β-galactosidase gene. The X-gal- positive stable cell line RSV-M/BAG was obtained by the FDG-FACS method. To examine the behavior of glioma cells in the brain, we homografted RSV-M/BAG cells into the brain of C3H/HeN mice as cell suspensions. Individual grafted glioma cells were easily detected by histochemical staining for B- galactosidase (β-gal). Three days after grafting, the β-gal-positive cells were mainly found in the subependymal zone of the lateral ventricle. In addition, some solitary labeled cells were found at locations distant from the injection sites. On the seventh day after implantation, tumor masses were observed and graft-derived glioma cells were migrating bilaterally along the fibers in the corpus callosum. Other labeled cells extended into the brain parenchyma via the perivascular (Virchow-Robin) spaces. Rapid and extensive migration of individual glioma cells was thus clearly demonstrated by intracerebral transplantation of RSV-M/BAG cells.",
keywords = "glioma, migration, retroviral vector, transplantation",
author = "M. Yamada and K. Shimizu and Y. Miyao and T. Hayakawa and Kazunori Nakajima and K. Nakahira and H. Nakagawa and K. Mikoshiba and K. Ikenaka",
year = "1994",
doi = "10.1002/jnr.490380407",
language = "English",
volume = "38",
pages = "415--423",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Migration of genetically labeled glioma cells after implantation into murine brain

AU - Yamada, M.

AU - Shimizu, K.

AU - Miyao, Y.

AU - Hayakawa, T.

AU - Nakajima, Kazunori

AU - Nakahira, K.

AU - Nakagawa, H.

AU - Mikoshiba, K.

AU - Ikenaka, K.

PY - 1994

Y1 - 1994

N2 - Murine RSV-M glioma cells were genetically labeled with a retroviral BAG vector carrying the Escherichia coli β-galactosidase gene. The X-gal- positive stable cell line RSV-M/BAG was obtained by the FDG-FACS method. To examine the behavior of glioma cells in the brain, we homografted RSV-M/BAG cells into the brain of C3H/HeN mice as cell suspensions. Individual grafted glioma cells were easily detected by histochemical staining for B- galactosidase (β-gal). Three days after grafting, the β-gal-positive cells were mainly found in the subependymal zone of the lateral ventricle. In addition, some solitary labeled cells were found at locations distant from the injection sites. On the seventh day after implantation, tumor masses were observed and graft-derived glioma cells were migrating bilaterally along the fibers in the corpus callosum. Other labeled cells extended into the brain parenchyma via the perivascular (Virchow-Robin) spaces. Rapid and extensive migration of individual glioma cells was thus clearly demonstrated by intracerebral transplantation of RSV-M/BAG cells.

AB - Murine RSV-M glioma cells were genetically labeled with a retroviral BAG vector carrying the Escherichia coli β-galactosidase gene. The X-gal- positive stable cell line RSV-M/BAG was obtained by the FDG-FACS method. To examine the behavior of glioma cells in the brain, we homografted RSV-M/BAG cells into the brain of C3H/HeN mice as cell suspensions. Individual grafted glioma cells were easily detected by histochemical staining for B- galactosidase (β-gal). Three days after grafting, the β-gal-positive cells were mainly found in the subependymal zone of the lateral ventricle. In addition, some solitary labeled cells were found at locations distant from the injection sites. On the seventh day after implantation, tumor masses were observed and graft-derived glioma cells were migrating bilaterally along the fibers in the corpus callosum. Other labeled cells extended into the brain parenchyma via the perivascular (Virchow-Robin) spaces. Rapid and extensive migration of individual glioma cells was thus clearly demonstrated by intracerebral transplantation of RSV-M/BAG cells.

KW - glioma

KW - migration

KW - retroviral vector

KW - transplantation

UR - http://www.scopus.com/inward/record.url?scp=0028237691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028237691&partnerID=8YFLogxK

U2 - 10.1002/jnr.490380407

DO - 10.1002/jnr.490380407

M3 - Article

C2 - 7932873

AN - SCOPUS:0028237691

VL - 38

SP - 415

EP - 423

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 4

ER -