Mikulicz's disease and Sjogren's syndrome

Kazuo Tsubota, Hiromi Fujita, Kensei Tsuzaka, Tsutomu Takeuchi

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

PURPOSE. To characterize lacrimal gland function and lymphocyte infiltration in patients with Mikulicz's disease (MD) and Sjogren's syndrome (SS). METHODS. Four patients with MD and 5 with SS were recruited, on whom were performed Schirmer test I (Schirmer test without anesthesia), Schirmer test with nasal stimulation, and vital staining of the ocular surface. The lacrimal gland was then biopsied and the tissues stained with CD3, CD4, CD8, B220, APO2.7, Fas, and Fas ligand (Fas-L) antibodies. RESULTS. Although regular Schirmer test results in the MD group were less than 10 mm, those with nasal stimulation, 38.1 ± 3.4 mm, were significantly greater than the SS group. There were minimal ocular surface changes in MD. Morphologic staining with hematoxylin and eosin was identical in both groups, but the acinar cells were stained with APO2.7 only in the SS group. There was strong Fas and Fas-L staining in SS patients but not in those with MD. CONCLUSIONS. Lacrimal gland acinar cells in those with MD maintained their function and were not programmed for cell death. The sicca syndrome was not observed in MD patients. Although the pathology is similar for MD and SS, the difference in acinar cell apoptosis and function can explain clinical differences.

Original languageEnglish
Pages (from-to)1666-1673
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number7
Publication statusPublished - 2000

Fingerprint

Mikulicz' Disease
Sjogren's Syndrome
Lacrimal Apparatus
Acinar Cells
Fas Ligand Protein
Staining and Labeling
Nose
Hematoxylin
Eosine Yellowish-(YS)
Cell Death
Anesthesia
Lymphocytes
Apoptosis
Pathology

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Mikulicz's disease and Sjogren's syndrome. / Tsubota, Kazuo; Fujita, Hiromi; Tsuzaka, Kensei; Takeuchi, Tsutomu.

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 7, 2000, p. 1666-1673.

Research output: Contribution to journalArticle

Tsubota, Kazuo ; Fujita, Hiromi ; Tsuzaka, Kensei ; Takeuchi, Tsutomu. / Mikulicz's disease and Sjogren's syndrome. In: Investigative Ophthalmology and Visual Science. 2000 ; Vol. 41, No. 7. pp. 1666-1673.
@article{af8a89ee8b424b38a3b51175eb8523ee,
title = "Mikulicz's disease and Sjogren's syndrome",
abstract = "PURPOSE. To characterize lacrimal gland function and lymphocyte infiltration in patients with Mikulicz's disease (MD) and Sjogren's syndrome (SS). METHODS. Four patients with MD and 5 with SS were recruited, on whom were performed Schirmer test I (Schirmer test without anesthesia), Schirmer test with nasal stimulation, and vital staining of the ocular surface. The lacrimal gland was then biopsied and the tissues stained with CD3, CD4, CD8, B220, APO2.7, Fas, and Fas ligand (Fas-L) antibodies. RESULTS. Although regular Schirmer test results in the MD group were less than 10 mm, those with nasal stimulation, 38.1 ± 3.4 mm, were significantly greater than the SS group. There were minimal ocular surface changes in MD. Morphologic staining with hematoxylin and eosin was identical in both groups, but the acinar cells were stained with APO2.7 only in the SS group. There was strong Fas and Fas-L staining in SS patients but not in those with MD. CONCLUSIONS. Lacrimal gland acinar cells in those with MD maintained their function and were not programmed for cell death. The sicca syndrome was not observed in MD patients. Although the pathology is similar for MD and SS, the difference in acinar cell apoptosis and function can explain clinical differences.",
author = "Kazuo Tsubota and Hiromi Fujita and Kensei Tsuzaka and Tsutomu Takeuchi",
year = "2000",
language = "English",
volume = "41",
pages = "1666--1673",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "7",

}

TY - JOUR

T1 - Mikulicz's disease and Sjogren's syndrome

AU - Tsubota, Kazuo

AU - Fujita, Hiromi

AU - Tsuzaka, Kensei

AU - Takeuchi, Tsutomu

PY - 2000

Y1 - 2000

N2 - PURPOSE. To characterize lacrimal gland function and lymphocyte infiltration in patients with Mikulicz's disease (MD) and Sjogren's syndrome (SS). METHODS. Four patients with MD and 5 with SS were recruited, on whom were performed Schirmer test I (Schirmer test without anesthesia), Schirmer test with nasal stimulation, and vital staining of the ocular surface. The lacrimal gland was then biopsied and the tissues stained with CD3, CD4, CD8, B220, APO2.7, Fas, and Fas ligand (Fas-L) antibodies. RESULTS. Although regular Schirmer test results in the MD group were less than 10 mm, those with nasal stimulation, 38.1 ± 3.4 mm, were significantly greater than the SS group. There were minimal ocular surface changes in MD. Morphologic staining with hematoxylin and eosin was identical in both groups, but the acinar cells were stained with APO2.7 only in the SS group. There was strong Fas and Fas-L staining in SS patients but not in those with MD. CONCLUSIONS. Lacrimal gland acinar cells in those with MD maintained their function and were not programmed for cell death. The sicca syndrome was not observed in MD patients. Although the pathology is similar for MD and SS, the difference in acinar cell apoptosis and function can explain clinical differences.

AB - PURPOSE. To characterize lacrimal gland function and lymphocyte infiltration in patients with Mikulicz's disease (MD) and Sjogren's syndrome (SS). METHODS. Four patients with MD and 5 with SS were recruited, on whom were performed Schirmer test I (Schirmer test without anesthesia), Schirmer test with nasal stimulation, and vital staining of the ocular surface. The lacrimal gland was then biopsied and the tissues stained with CD3, CD4, CD8, B220, APO2.7, Fas, and Fas ligand (Fas-L) antibodies. RESULTS. Although regular Schirmer test results in the MD group were less than 10 mm, those with nasal stimulation, 38.1 ± 3.4 mm, were significantly greater than the SS group. There were minimal ocular surface changes in MD. Morphologic staining with hematoxylin and eosin was identical in both groups, but the acinar cells were stained with APO2.7 only in the SS group. There was strong Fas and Fas-L staining in SS patients but not in those with MD. CONCLUSIONS. Lacrimal gland acinar cells in those with MD maintained their function and were not programmed for cell death. The sicca syndrome was not observed in MD patients. Although the pathology is similar for MD and SS, the difference in acinar cell apoptosis and function can explain clinical differences.

UR - http://www.scopus.com/inward/record.url?scp=0034130613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034130613&partnerID=8YFLogxK

M3 - Article

C2 - 10845583

AN - SCOPUS:0034130613

VL - 41

SP - 1666

EP - 1673

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 7

ER -