Mini-Gut Organoids

Reconstitution of the Stem Cell Niche

Shoichi Date, Toshiro Sato

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.

Original languageEnglish
Pages (from-to)269-289
Number of pages21
JournalAnnual Review of Cell and Developmental Biology
Volume31
DOIs
Publication statusPublished - 2015 Nov 13

Fingerprint

Organoids
Stem Cell Niche
Stem Cells
Carcinogenesis
Epithelium
Digestive System
Regenerative Medicine
Organogenesis
Genetic Models
Epidermal Growth Factor
Growth
Cell Self Renewal

Keywords

  • Cancer
  • Intestine
  • Liver
  • Niche
  • Organoids
  • Pancreas
  • Stem cells
  • Stomach

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Mini-Gut Organoids : Reconstitution of the Stem Cell Niche. / Date, Shoichi; Sato, Toshiro.

In: Annual Review of Cell and Developmental Biology, Vol. 31, 13.11.2015, p. 269-289.

Research output: Contribution to journalArticle

@article{cc9e00925c8b4fcc81da764fa79bc38e,
title = "Mini-Gut Organoids: Reconstitution of the Stem Cell Niche",
abstract = "In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.",
keywords = "Cancer, Intestine, Liver, Niche, Organoids, Pancreas, Stem cells, Stomach",
author = "Shoichi Date and Toshiro Sato",
year = "2015",
month = "11",
day = "13",
doi = "10.1146/annurev-cellbio-100814-125218",
language = "English",
volume = "31",
pages = "269--289",
journal = "Annual Review of Cell and Developmental Biology",
issn = "1081-0706",
publisher = "Annual Reviews Inc.",

}

TY - JOUR

T1 - Mini-Gut Organoids

T2 - Reconstitution of the Stem Cell Niche

AU - Date, Shoichi

AU - Sato, Toshiro

PY - 2015/11/13

Y1 - 2015/11/13

N2 - In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.

AB - In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.

KW - Cancer

KW - Intestine

KW - Liver

KW - Niche

KW - Organoids

KW - Pancreas

KW - Stem cells

KW - Stomach

UR - http://www.scopus.com/inward/record.url?scp=84947207751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947207751&partnerID=8YFLogxK

U2 - 10.1146/annurev-cellbio-100814-125218

DO - 10.1146/annurev-cellbio-100814-125218

M3 - Article

VL - 31

SP - 269

EP - 289

JO - Annual Review of Cell and Developmental Biology

JF - Annual Review of Cell and Developmental Biology

SN - 1081-0706

ER -