Abstract
In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.
| Original language | English |
|---|---|
| Pages (from-to) | 269-289 |
| Number of pages | 21 |
| Journal | Annual Review of Cell and Developmental Biology |
| Volume | 31 |
| DOIs | |
| Publication status | Published - 2015 Nov 13 |
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Keywords
- Cancer
- Intestine
- Liver
- Niche
- Organoids
- Pancreas
- Stem cells
- Stomach
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Mini-Gut Organoids : Reconstitution of the Stem Cell Niche. / Date, Shoichi; Sato, Toshiro.
In: Annual Review of Cell and Developmental Biology, Vol. 31, 13.11.2015, p. 269-289.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mini-Gut Organoids
T2 - Reconstitution of the Stem Cell Niche
AU - Date, Shoichi
AU - Sato, Toshiro
PY - 2015/11/13
Y1 - 2015/11/13
N2 - In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.
AB - In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMPTGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5+ stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.
KW - Cancer
KW - Intestine
KW - Liver
KW - Niche
KW - Organoids
KW - Pancreas
KW - Stem cells
KW - Stomach
UR - http://www.scopus.com/inward/record.url?scp=84947207751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84947207751&partnerID=8YFLogxK
U2 - 10.1146/annurev-cellbio-100814-125218
DO - 10.1146/annurev-cellbio-100814-125218
M3 - Article
C2 - 26436704
AN - SCOPUS:84947207751
VL - 31
SP - 269
EP - 289
JO - Annual Review of Cell and Developmental Biology
JF - Annual Review of Cell and Developmental Biology
SN - 1081-0706
ER -