mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity

Marcin Łyszkiewicz, Samantha J. Winter, Katrin Witzlau, Lisa Föhse, Rebecca Brownlie, Jacek Puchałka, Nikita A. Verheyden, Heike Kunze-Schumacher, Esther Imelmann, Jonas Blume, Solaiman Raha, Takashi Sekiya, Akihiko Yoshimura, Jochen T. Frueh, Evelyn Ullrich, Jochen Huehn, Siegfried Weiss, Maximiliano G. Gutierrez, Immo Prinz, Rose ZamoyskaNatalia Ziętara, Andreas Krueger

Research output: Contribution to journalArticle

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Abstract

The interdependence of selective cues during development of regulatory T cells (Treg cells) in the thymus and their suppressive function remains incompletely understood. Here, we analyzed this interdependence by taking advantage of highly dynamic changes in expression of microRNA 181 family members miR-181a-1 and miR-181b-1 (miR-181a/b-1) during late T-cell development with very high levels of expression during thymocyte selection, followed by massive down-regulation in the periphery. Loss of miR-181a/b-1 resulted in inefficient de novo generation of Treg cells in the thymus but simultaneously permitted homeostatic expansion in the periphery in the absence of competition. Modulation of T-cell receptor (TCR) signal strength in vivo indicated that miR-181a/b-1 controlled Treg-cell formation via establishing adequate signaling thresholds. Unexpectedly, miR-181a/b-1–deficient Treg cells displayed elevated suppressive capacity in vivo, in line with elevated levels of cytotoxic T-lymphocyte–associated 4 (CTLA-4) protein, but not mRNA, in thymic and peripheral Treg cells. Therefore, we propose that intrathymic miR-181a/b-1 controls development of Treg cells and imposes a developmental legacy on their peripheral function.

Original languageEnglish
Article numbere2006716
JournalPLoS Biology
Volume17
Issue number3
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

Thymus
T-cells
Regulatory T-Lymphocytes
T-Cell Antigen Receptor
MicroRNAs
T-lymphocytes
Modulation
cells
Messenger RNA
Thymus Gland
Proteins
thymocytes
microRNA
Thymocytes
Cues
Down-Regulation
receptors
T-Lymphocytes
proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Łyszkiewicz, M., Winter, S. J., Witzlau, K., Föhse, L., Brownlie, R., Puchałka, J., ... Krueger, A. (2019). mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity. PLoS Biology, 17(3), [e2006716]. https://doi.org/10.1371/journal.pbio.2006716

mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity. / Łyszkiewicz, Marcin; Winter, Samantha J.; Witzlau, Katrin; Föhse, Lisa; Brownlie, Rebecca; Puchałka, Jacek; Verheyden, Nikita A.; Kunze-Schumacher, Heike; Imelmann, Esther; Blume, Jonas; Raha, Solaiman; Sekiya, Takashi; Yoshimura, Akihiko; Frueh, Jochen T.; Ullrich, Evelyn; Huehn, Jochen; Weiss, Siegfried; Gutierrez, Maximiliano G.; Prinz, Immo; Zamoyska, Rose; Ziętara, Natalia; Krueger, Andreas.

In: PLoS Biology, Vol. 17, No. 3, e2006716, 01.03.2019.

Research output: Contribution to journalArticle

Łyszkiewicz, M, Winter, SJ, Witzlau, K, Föhse, L, Brownlie, R, Puchałka, J, Verheyden, NA, Kunze-Schumacher, H, Imelmann, E, Blume, J, Raha, S, Sekiya, T, Yoshimura, A, Frueh, JT, Ullrich, E, Huehn, J, Weiss, S, Gutierrez, MG, Prinz, I, Zamoyska, R, Ziętara, N & Krueger, A 2019, 'mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity', PLoS Biology, vol. 17, no. 3, e2006716. https://doi.org/10.1371/journal.pbio.2006716
Łyszkiewicz M, Winter SJ, Witzlau K, Föhse L, Brownlie R, Puchałka J et al. mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity. PLoS Biology. 2019 Mar 1;17(3). e2006716. https://doi.org/10.1371/journal.pbio.2006716
Łyszkiewicz, Marcin ; Winter, Samantha J. ; Witzlau, Katrin ; Föhse, Lisa ; Brownlie, Rebecca ; Puchałka, Jacek ; Verheyden, Nikita A. ; Kunze-Schumacher, Heike ; Imelmann, Esther ; Blume, Jonas ; Raha, Solaiman ; Sekiya, Takashi ; Yoshimura, Akihiko ; Frueh, Jochen T. ; Ullrich, Evelyn ; Huehn, Jochen ; Weiss, Siegfried ; Gutierrez, Maximiliano G. ; Prinz, Immo ; Zamoyska, Rose ; Ziętara, Natalia ; Krueger, Andreas. / mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity. In: PLoS Biology. 2019 ; Vol. 17, No. 3.
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