MiR-200b and miR-429 function in mouse ovulation and are essential for female fertility

Hidetoshi Hasuwa, Jun Ueda, Masahito Ikawa, Masaru Okabe

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Ovulation in the mouse and other mammals is controlled by hormones secreted by the hypothalamo-pituitary-ovarian axis. We describe anovulation and infertility in female mice lacking the microRNAs miR-200b and miR-429. Both miRNAs are strongly expressed in the pituitary gland, where they suppress expression of the transcriptional repressor ZEB1. Eliminating these miRNAs, in turn, inhibits luteinizing hormone (LH) synthesis by repressing transcription of its β-subunit gene, which leads to lowered serum LH concentration, an impaired LH surge, and failure to ovulate. Our results reveal roles for miR-200b and miR-429, and their target the Zeb1 gene, in the regulation of mammalian reproduction. Thus, the hypothalamo-pituitary-ovarian axis was shown to require miR-200b and miR-429 to support ovulation.

Original languageEnglish
Pages (from-to)71-73
Number of pages3
JournalScience
Volume341
Issue number6141
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Luteinizing Hormone
Ovulation
MicroRNAs
Fertility
Anovulation
Female Infertility
Pituitary Gland
Genes
Reproduction
Mammals
Hormones
Serum

ASJC Scopus subject areas

  • General

Cite this

MiR-200b and miR-429 function in mouse ovulation and are essential for female fertility. / Hasuwa, Hidetoshi; Ueda, Jun; Ikawa, Masahito; Okabe, Masaru.

In: Science, Vol. 341, No. 6141, 2013, p. 71-73.

Research output: Contribution to journalArticle

Hasuwa, Hidetoshi ; Ueda, Jun ; Ikawa, Masahito ; Okabe, Masaru. / MiR-200b and miR-429 function in mouse ovulation and are essential for female fertility. In: Science. 2013 ; Vol. 341, No. 6141. pp. 71-73.
@article{b090fadc381340ab93d3a20d44903b47,
title = "MiR-200b and miR-429 function in mouse ovulation and are essential for female fertility",
abstract = "Ovulation in the mouse and other mammals is controlled by hormones secreted by the hypothalamo-pituitary-ovarian axis. We describe anovulation and infertility in female mice lacking the microRNAs miR-200b and miR-429. Both miRNAs are strongly expressed in the pituitary gland, where they suppress expression of the transcriptional repressor ZEB1. Eliminating these miRNAs, in turn, inhibits luteinizing hormone (LH) synthesis by repressing transcription of its β-subunit gene, which leads to lowered serum LH concentration, an impaired LH surge, and failure to ovulate. Our results reveal roles for miR-200b and miR-429, and their target the Zeb1 gene, in the regulation of mammalian reproduction. Thus, the hypothalamo-pituitary-ovarian axis was shown to require miR-200b and miR-429 to support ovulation.",
author = "Hidetoshi Hasuwa and Jun Ueda and Masahito Ikawa and Masaru Okabe",
year = "2013",
doi = "10.1126/science.1237999",
language = "English",
volume = "341",
pages = "71--73",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6141",

}

TY - JOUR

T1 - MiR-200b and miR-429 function in mouse ovulation and are essential for female fertility

AU - Hasuwa, Hidetoshi

AU - Ueda, Jun

AU - Ikawa, Masahito

AU - Okabe, Masaru

PY - 2013

Y1 - 2013

N2 - Ovulation in the mouse and other mammals is controlled by hormones secreted by the hypothalamo-pituitary-ovarian axis. We describe anovulation and infertility in female mice lacking the microRNAs miR-200b and miR-429. Both miRNAs are strongly expressed in the pituitary gland, where they suppress expression of the transcriptional repressor ZEB1. Eliminating these miRNAs, in turn, inhibits luteinizing hormone (LH) synthesis by repressing transcription of its β-subunit gene, which leads to lowered serum LH concentration, an impaired LH surge, and failure to ovulate. Our results reveal roles for miR-200b and miR-429, and their target the Zeb1 gene, in the regulation of mammalian reproduction. Thus, the hypothalamo-pituitary-ovarian axis was shown to require miR-200b and miR-429 to support ovulation.

AB - Ovulation in the mouse and other mammals is controlled by hormones secreted by the hypothalamo-pituitary-ovarian axis. We describe anovulation and infertility in female mice lacking the microRNAs miR-200b and miR-429. Both miRNAs are strongly expressed in the pituitary gland, where they suppress expression of the transcriptional repressor ZEB1. Eliminating these miRNAs, in turn, inhibits luteinizing hormone (LH) synthesis by repressing transcription of its β-subunit gene, which leads to lowered serum LH concentration, an impaired LH surge, and failure to ovulate. Our results reveal roles for miR-200b and miR-429, and their target the Zeb1 gene, in the regulation of mammalian reproduction. Thus, the hypothalamo-pituitary-ovarian axis was shown to require miR-200b and miR-429 to support ovulation.

UR - http://www.scopus.com/inward/record.url?scp=84879796694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879796694&partnerID=8YFLogxK

U2 - 10.1126/science.1237999

DO - 10.1126/science.1237999

M3 - Article

VL - 341

SP - 71

EP - 73

JO - Science

JF - Science

SN - 0036-8075

IS - 6141

ER -