miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

Ryuji Morizane; Shizuka Fujii; Toshiaki Monkawa; Ken Hiratsuka; Shintaro Yamaguchi; Koichiro Homma; Hiroshi Itoh

doi:10.1007/s10157-015-1167-2

miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

Ryuji Morizane, Shizuka Fujii, Toshiaki Monkawa, Ken Hiratsuka, Shintaro Yamaguchi, Koichiro Homma, Hiroshi Itoh

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

Original language	English
Pages (from-to)	394-401
Number of pages	8
Journal	Clinical and experimental nephrology
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/s10157-015-1167-2
Publication status	Published - 2016 Jun 1

Keywords

Cancer
EMT
Fibrosis
Kidney
miR-363
miRNA

ASJC Scopus subject areas

Physiology
Nephrology
Physiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10157-015-1167-2

Cite this

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title = "miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype",

abstract = "Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.",

keywords = "Cancer, EMT, Fibrosis, Kidney, miR-363, miRNA",

author = "Ryuji Morizane and Shizuka Fujii and Toshiaki Monkawa and Ken Hiratsuka and Shintaro Yamaguchi and Koichiro Homma and Hiroshi Itoh",

note = "Publisher Copyright: {\textcopyright} 2015, Japanese Society of Nephrology.",

year = "2016",

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day = "1",

doi = "10.1007/s10157-015-1167-2",

language = "English",

volume = "20",

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TY - JOUR

T1 - miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

AU - Morizane, Ryuji

AU - Fujii, Shizuka

AU - Monkawa, Toshiaki

AU - Hiratsuka, Ken

AU - Yamaguchi, Shintaro

AU - Homma, Koichiro

AU - Itoh, Hiroshi

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

AB - Background: microRNAs (miRNAs) are non-coding small RNAs that regulate embryonic development, cell differentiation and pathological processes via interaction with mRNA. Epithelial–mesenchymal transition (EMT) is pathological process that involves in a variety of diseases such as cancer or fibrosis. Methods: In this study, we identified miR-363 as a potent inducer of EMT by microarray analysis in human kidney tubular cells, and analyzed the function and mechanisms of miR-363. Results: Overexpression of miR-363 induced mesenchymal phenotypes with loss of epithelial phenotypes in human kidney tubular cells. In addition, in vitro scratch assay demonstrated that miR-363 promotes cell migration of primary culture of human kidney tubular cells. We identified TWIST/canonical WNT pathway as the downstream effecter of miR-363, and inhibition of canonical WNT by small molecule, IWR-1, attenuated EMT induced by miR-363. Conclusion: miR-363 induces transdifferentiation of human kidney tubular cells via upregulation of TWIST/canonical WNT pathway.

KW - Cancer

KW - EMT

KW - Fibrosis

KW - Kidney

KW - miR-363

KW - miRNA

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U2 - 10.1007/s10157-015-1167-2

DO - 10.1007/s10157-015-1167-2

M3 - Article

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AN - SCOPUS:84941702722

SN - 1342-1751

VL - 20

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JO - Clinical and Experimental Nephrology

JF - Clinical and Experimental Nephrology

IS - 3

ER -

miR-363 induces transdifferentiation of human kidney tubular cells to mesenchymal phenotype

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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