Mitigation of occult lung injury by pneumonectomy via minithoracotomy in mice

M. Kohno, Masazumi Watanabe, Y. Izumi, S. Tasaka, Yuukou Kitagawa, I. Maruyama, K. Kobayashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background The systemic and pulmonary inflammatory response associated with pneumonectomy performed via minithoracotomy versus that after open posterolateral thoracotomy is uncertain. Methods Groups consisting of 7 randomly assigned mice underwent a) minithoracotomy (with 5-mm long incisions and sparing of the muscles) alone, b) posterolateral thoracotomy (with 20-mm long incisions) alone, c) pneumonectomy via minithoracotomy, or d) pneumonectomy via posterolateral thoracotomy. The animals' daily food intake, body weight changes and spontaneous activity were monitored for 10 days, and lung water accumulation and vascular hyperpermeability in the remaining right lung were measured at 24 h after surgery. Concentrations of high mobility group box 1 protein (HMGB1), a mediator of inflammation and shock, were measured in the bronchoalveolar lavage fluid. Results Compared with posterolateral thoracotomy, pneumonectomy via minithoracotomy was associated with significantly less weight loss (p < 0.05), despite a similar daily food intake among the groups. Spontaneous activity after pneumonectomy via minithoracotomy returned earlier than after posterolateral thoracotomy. Pulmonary vascular hyperpermeability and water retention in the residual lung were significantly less prominent after pneumonectomy performed via minithoracotomy than after pneumonectomy via posterolateral thoracotomy (both comparisons p < 0.05). HMGB1 concentrations in the bronchoalveolar lavage fluid collected from the residual lung were significantly lower (p < 0.05) after minithoracotomy than after posterolateral thoracotomy. Conclusions Based on postoperative weight loss, spontaneous activity, and the degree of pulmonary capillary injury in the residual lung, pneumonectomy via minithoracotomy was less invasive than posterolateral thoracotomy. The lower increase in HMGB1 associated with minithoracotomy might result in lower pulmonary vascular hyperpermeability and reflect less surgical invasiveness.

Original languageEnglish
Pages (from-to)124-130
Number of pages7
JournalThoracic and Cardiovascular Surgeon
Volume60
Issue number2
DOIs
Publication statusPublished - 2012

Fingerprint

Pneumonectomy
Lung Injury
Thoracotomy
Lung
HMGB1 Protein
Blood Vessels
Bronchoalveolar Lavage Fluid
Weight Loss
Eating
Inflammation Mediators
Body Weight Changes
Water
Shock
Muscles

Keywords

  • inflammation systemic (incl. cells, mediators)
  • lung cancer treatment (surgery, medical)
  • minimally invasive surgery (includes port access minithoracotomy)
  • surgery/incisions
  • thoracoscopy/VATS

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

Cite this

Mitigation of occult lung injury by pneumonectomy via minithoracotomy in mice. / Kohno, M.; Watanabe, Masazumi; Izumi, Y.; Tasaka, S.; Kitagawa, Yuukou; Maruyama, I.; Kobayashi, K.

In: Thoracic and Cardiovascular Surgeon, Vol. 60, No. 2, 2012, p. 124-130.

Research output: Contribution to journalArticle

Kohno, M, Watanabe, M, Izumi, Y, Tasaka, S, Kitagawa, Y, Maruyama, I & Kobayashi, K 2012, 'Mitigation of occult lung injury by pneumonectomy via minithoracotomy in mice', Thoracic and Cardiovascular Surgeon, vol. 60, no. 2, pp. 124-130. https://doi.org/10.1055/s-0030-1271011
Kohno, M. ; Watanabe, Masazumi ; Izumi, Y. ; Tasaka, S. ; Kitagawa, Yuukou ; Maruyama, I. ; Kobayashi, K. / Mitigation of occult lung injury by pneumonectomy via minithoracotomy in mice. In: Thoracic and Cardiovascular Surgeon. 2012 ; Vol. 60, No. 2. pp. 124-130.
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AB - Background The systemic and pulmonary inflammatory response associated with pneumonectomy performed via minithoracotomy versus that after open posterolateral thoracotomy is uncertain. Methods Groups consisting of 7 randomly assigned mice underwent a) minithoracotomy (with 5-mm long incisions and sparing of the muscles) alone, b) posterolateral thoracotomy (with 20-mm long incisions) alone, c) pneumonectomy via minithoracotomy, or d) pneumonectomy via posterolateral thoracotomy. The animals' daily food intake, body weight changes and spontaneous activity were monitored for 10 days, and lung water accumulation and vascular hyperpermeability in the remaining right lung were measured at 24 h after surgery. Concentrations of high mobility group box 1 protein (HMGB1), a mediator of inflammation and shock, were measured in the bronchoalveolar lavage fluid. Results Compared with posterolateral thoracotomy, pneumonectomy via minithoracotomy was associated with significantly less weight loss (p < 0.05), despite a similar daily food intake among the groups. Spontaneous activity after pneumonectomy via minithoracotomy returned earlier than after posterolateral thoracotomy. Pulmonary vascular hyperpermeability and water retention in the residual lung were significantly less prominent after pneumonectomy performed via minithoracotomy than after pneumonectomy via posterolateral thoracotomy (both comparisons p < 0.05). HMGB1 concentrations in the bronchoalveolar lavage fluid collected from the residual lung were significantly lower (p < 0.05) after minithoracotomy than after posterolateral thoracotomy. Conclusions Based on postoperative weight loss, spontaneous activity, and the degree of pulmonary capillary injury in the residual lung, pneumonectomy via minithoracotomy was less invasive than posterolateral thoracotomy. The lower increase in HMGB1 associated with minithoracotomy might result in lower pulmonary vascular hyperpermeability and reflect less surgical invasiveness.

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