Mitosis in beating cardiac muscle cells from newt embryos in vitro

Hiroyuki Kaneko, S. I. Abe, S. Ito

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cardiac muscle cells from newt embryos were cultured at relatively low cell density. Within 10 days in culture, 2 cell types (spindle and flat type) were distinguished both among beating and non-beating cells. Mitosis in single beating cells was frequently observed both in spindle and flat cells. Some cells maintained almost constant contractile activities throughout the mitotic stages, while the others transiently stopped beating during mitosis, which accords well to the case in chick embryos. Ultra-thin section shows the presence of myofibril's structure in a dividing cell, as shown in newborn rats, chick embryos and adult newts. As a consequence of mitosis, 3 types (spindle, flat and mixed type) of beating colonies developed after 3-4 weeks in culture. Cell proliferation was accompanied by pulsation and could be directly pursued till the 4th division, suggesting that differentiated myocardiac cells with myofibrils proliferate by their mitoses in vivo, maintaining rhythmic contraction.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalDevelopment Growth and Differentiation
Volume23
Issue number3
Publication statusPublished - 1981
Externally publishedYes

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Salamandridae
Mitosis
Cardiac Myocytes
Embryonic Structures
Myofibrils
Chick Embryo
In Vitro Techniques
Cell Culture Techniques
Cell Count
Cell Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy

Cite this

Mitosis in beating cardiac muscle cells from newt embryos in vitro. / Kaneko, Hiroyuki; Abe, S. I.; Ito, S.

In: Development Growth and Differentiation, Vol. 23, No. 3, 1981, p. 237-244.

Research output: Contribution to journalArticle

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