Mitral cells in the olfactory bulb are mainly excited through a multistep signaling path

David H. Gire, Kevin M. Franks, Joseph D. Zak, Kenji Tanaka, Jennifer D. Whitesell, Abigail A. Mulligan, René Hen, Nathan E. Schoppa

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Within the olfactory system, information flow from the periphery onto output mitral cells (MCs) of the olfactory bulb (OB) has been thought to be mediated by direct synaptic inputs from olfactory sensory neurons (OSNs). Here, we performed patch-clamp measurements in rat and mouse OB slices to investigate mechanisms of OSN signaling onto MCs, including the assumption of a direct path, using electrical and optogenetic stimulation methods that selectively activated OSNs. We found that MCs are in fact not typically activated by direct OSN inputs and instead require a multistep, diffuse mechanism involving another glutamatergic cell type, the tufted cells. The preference for a multistep mechanism reflects the fact that signals arising from direct OSN inputs are drastically shunted by connexin 36-mediated gap junctions on MCs, but not tufted cells. An OB circuit with tufted cells intermediate between OSNs and MCs suggests that considerable processing of olfactory information occurs before its reaching MCs.

Original languageEnglish
Pages (from-to)2964-2975
Number of pages12
JournalJournal of Neuroscience
Volume32
Issue number9
DOIs
Publication statusPublished - 2012 Feb 29
Externally publishedYes

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Olfactory Bulb
Olfactory Receptor Neurons
Optogenetics
Gap Junctions
Automatic Data Processing
Electric Stimulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Gire, D. H., Franks, K. M., Zak, J. D., Tanaka, K., Whitesell, J. D., Mulligan, A. A., ... Schoppa, N. E. (2012). Mitral cells in the olfactory bulb are mainly excited through a multistep signaling path. Journal of Neuroscience, 32(9), 2964-2975. https://doi.org/10.1523/JNEUROSCI.5580-11.2012

Mitral cells in the olfactory bulb are mainly excited through a multistep signaling path. / Gire, David H.; Franks, Kevin M.; Zak, Joseph D.; Tanaka, Kenji; Whitesell, Jennifer D.; Mulligan, Abigail A.; Hen, René; Schoppa, Nathan E.

In: Journal of Neuroscience, Vol. 32, No. 9, 29.02.2012, p. 2964-2975.

Research output: Contribution to journalArticle

Gire, DH, Franks, KM, Zak, JD, Tanaka, K, Whitesell, JD, Mulligan, AA, Hen, R & Schoppa, NE 2012, 'Mitral cells in the olfactory bulb are mainly excited through a multistep signaling path', Journal of Neuroscience, vol. 32, no. 9, pp. 2964-2975. https://doi.org/10.1523/JNEUROSCI.5580-11.2012
Gire, David H. ; Franks, Kevin M. ; Zak, Joseph D. ; Tanaka, Kenji ; Whitesell, Jennifer D. ; Mulligan, Abigail A. ; Hen, René ; Schoppa, Nathan E. / Mitral cells in the olfactory bulb are mainly excited through a multistep signaling path. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 9. pp. 2964-2975.
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