Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine

Margaret L. Schwarze, Matthew T. Menard, Yasushi Fuchimoto, Christene A. Huang, Stuart Houser, Kwabena Mawulawde, Kenneth S. Allison, David H. Sachs, Joren C. Madsen

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background Tolerance to cardiac allografts has not been achieved in large animals using methods that are readily applicable to human recipients. We investigated the effects of mixed hematopoietic chimerism on cardiac allograft survival and chronic rejection in miniature swine. Methods. Recipients were T-cell depleted using a porcine CD3 immunotoxin, and each received either of two nonmyeloablative preparative regimens previously demonstrated to permit the establishment of stable mixed hematopoietic chimerism across MHC-matched, minor-antigen-mismatched histocompatibility barriers. Five to 12 months after the chimerism was induced, hearts from the original cell donors were heterotopically transplanted into the stable mixed chimeras. Results. Cardiac allografts transplanted into untreated recipients across similar minor antigen barriers were rejected within 44 days (within 21, 28, 35, 39, 44 days among individual study subjects). In contrast, hearts transplanted into the mixed chimeras were all accepted long term (> 153, > 225, > 286, > 362 days) without immunosuppressive drugs and developed minimal vasculopathy. Conclusions. Mixed hematopoietic chimerism, established in miniature swine using clinically relevant, nonmyeloablative conditioning regimens, permits long-term cardiac allograft survival without chronic immunosuppressive therapy, significant vasculopathy, or graft-versus-host disease.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalAnnals of Thoracic Surgery
Volume70
Issue number1
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Miniature Swine
Chimerism
Allografts
Immunosuppressive Agents
Minor Histocompatibility Antigens
Immunotoxins
Graft vs Host Disease
Swine
T-Lymphocytes
Antigens
Pharmaceutical Preparations
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Schwarze, M. L., Menard, M. T., Fuchimoto, Y., Huang, C. A., Houser, S., Mawulawde, K., ... Madsen, J. C. (2000). Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine. Annals of Thoracic Surgery, 70(1), 131-139. https://doi.org/10.1016/S0003-4975(00)01564-2

Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine. / Schwarze, Margaret L.; Menard, Matthew T.; Fuchimoto, Yasushi; Huang, Christene A.; Houser, Stuart; Mawulawde, Kwabena; Allison, Kenneth S.; Sachs, David H.; Madsen, Joren C.

In: Annals of Thoracic Surgery, Vol. 70, No. 1, 2000, p. 131-139.

Research output: Contribution to journalArticle

Schwarze, ML, Menard, MT, Fuchimoto, Y, Huang, CA, Houser, S, Mawulawde, K, Allison, KS, Sachs, DH & Madsen, JC 2000, 'Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine', Annals of Thoracic Surgery, vol. 70, no. 1, pp. 131-139. https://doi.org/10.1016/S0003-4975(00)01564-2
Schwarze, Margaret L. ; Menard, Matthew T. ; Fuchimoto, Yasushi ; Huang, Christene A. ; Houser, Stuart ; Mawulawde, Kwabena ; Allison, Kenneth S. ; Sachs, David H. ; Madsen, Joren C. / Mixed hematopoietic chimerism induces long-term tolerance to cardiac allografts in miniature swine. In: Annals of Thoracic Surgery. 2000 ; Vol. 70, No. 1. pp. 131-139.
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N2 - Background Tolerance to cardiac allografts has not been achieved in large animals using methods that are readily applicable to human recipients. We investigated the effects of mixed hematopoietic chimerism on cardiac allograft survival and chronic rejection in miniature swine. Methods. Recipients were T-cell depleted using a porcine CD3 immunotoxin, and each received either of two nonmyeloablative preparative regimens previously demonstrated to permit the establishment of stable mixed hematopoietic chimerism across MHC-matched, minor-antigen-mismatched histocompatibility barriers. Five to 12 months after the chimerism was induced, hearts from the original cell donors were heterotopically transplanted into the stable mixed chimeras. Results. Cardiac allografts transplanted into untreated recipients across similar minor antigen barriers were rejected within 44 days (within 21, 28, 35, 39, 44 days among individual study subjects). In contrast, hearts transplanted into the mixed chimeras were all accepted long term (> 153, > 225, > 286, > 362 days) without immunosuppressive drugs and developed minimal vasculopathy. Conclusions. Mixed hematopoietic chimerism, established in miniature swine using clinically relevant, nonmyeloablative conditioning regimens, permits long-term cardiac allograft survival without chronic immunosuppressive therapy, significant vasculopathy, or graft-versus-host disease.

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