Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1

Jun Kunisawa, Yuki Sugiura, Taichi Wake, Takahiro Nagatake, Hidehiko Suzuki, Risa Nagasawa, Shiori Shikata, Kurara Honda, Eri Hashimoto, Yuji Suzuki, Mitsutoshi Setou, Makoto Suematsu, Hiroshi Kiyono

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Bioenergetic metabolism varies during cell differentiation, but details of B cell metabolism remain unclear. Here, we show the metabolic changes during B cell differentiation in the intestine, where B cells differentiate into IgA+ plasma cells (PCs). Naive B cells in the Peyer's patches (PPs) and IgA+ PCs in the intestinal lamina propria (iLP) both used the tricarboxylic acid (TCA) cycle, but only IgA+ PCs underwent glycolysis. These metabolic differences reflected their dependencies on vitamin B1, an essential cofactor for the TCA cycle. Indeed, the diminished activity of the TCA cycle after dietary vitamin B1 depletion decreased the number of naive B cells in PPs without affecting IgA+ PCs in the iLP. The maintenance of naive B cells by dietary vitamin B1 was required to induce-but not maintain-intestinal IgA responses against oral antigens. These findings reveal the diet-mediated maintenance of B cell immunometabolism in organized and diffuse intestinal tissues.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalCell Reports
Volume13
Issue number1
DOIs
Publication statusPublished - 2015 Oct 6

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Thiamine
Metabolism
Energy Metabolism
Intestines
Cell Differentiation
B-Lymphocytes
Cells
Immunoglobulin A
Plasma Cells
Citric Acid Cycle
Plasmas
Peyer's Patches
Mucous Membrane
Maintenance
Glycolysis
Nutrition
Tissue
Diet
Antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1 . / Kunisawa, Jun; Sugiura, Yuki; Wake, Taichi; Nagatake, Takahiro; Suzuki, Hidehiko; Nagasawa, Risa; Shikata, Shiori; Honda, Kurara; Hashimoto, Eri; Suzuki, Yuji; Setou, Mitsutoshi; Suematsu, Makoto; Kiyono, Hiroshi.

In: Cell Reports, Vol. 13, No. 1, 06.10.2015, p. 122-131.

Research output: Contribution to journalArticle

Kunisawa, J, Sugiura, Y, Wake, T, Nagatake, T, Suzuki, H, Nagasawa, R, Shikata, S, Honda, K, Hashimoto, E, Suzuki, Y, Setou, M, Suematsu, M & Kiyono, H 2015, 'Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1 ', Cell Reports, vol. 13, no. 1, pp. 122-131. https://doi.org/10.1016/j.celrep.2015.08.063
Kunisawa, Jun ; Sugiura, Yuki ; Wake, Taichi ; Nagatake, Takahiro ; Suzuki, Hidehiko ; Nagasawa, Risa ; Shikata, Shiori ; Honda, Kurara ; Hashimoto, Eri ; Suzuki, Yuji ; Setou, Mitsutoshi ; Suematsu, Makoto ; Kiyono, Hiroshi. / Mode of Bioenergetic Metabolism during B Cell Differentiation in the Intestine Determines the Distinct Requirement for Vitamin B1 In: Cell Reports. 2015 ; Vol. 13, No. 1. pp. 122-131.
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