Model for paraaortic lymph node metastasis produced by orthotopic implantation of ovarian carcinoma cells in athymic nude mice

Y. Tamada, Daisuke Aoki, S. Nozawa, T. Irimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Lymph node metastasis through the lymphatic vessels is a critical step in determining the outcome of ovarian cancer patients, and prognosis should be improved by preventing lymph node metastasis. However, experimental models for lymph node metastasis of ovarian carcinoma are not available. We developed an orthotopic transplantation model to study this process in nude mice using the human ovarian carcinoma cell lines, KF and MH. Highly metastatic sublines (KF-LN3 and MH-LN3) were selected in vivo in nude mice by repeated orthotopic transplantation, lymph node metastasis formation and culturing the tumour cells in vitro. Because this model seems to correspond to the advanced clinical stage of ovarian carcinomas, it should be useful in understanding the molecular biology of ovarian carcinomas and in the development of therapeutic modalities against lymph node metastasis.

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalEuropean Journal of Cancer
Volume40
Issue number1
DOIs
Publication statusPublished - 2004 Jan

Fingerprint

Nude Mice
Lymph Nodes
Neoplasm Metastasis
Carcinoma
Transplantation
Lymphatic Vessels
Ovarian Neoplasms
Molecular Biology
Theoretical Models
Cell Line
Neoplasms
Therapeutics

Keywords

  • Animal model
  • Lymph node metastasis
  • Ovarian cancer

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Model for paraaortic lymph node metastasis produced by orthotopic implantation of ovarian carcinoma cells in athymic nude mice. / Tamada, Y.; Aoki, Daisuke; Nozawa, S.; Irimura, T.

In: European Journal of Cancer, Vol. 40, No. 1, 01.2004, p. 158-163.

Research output: Contribution to journalArticle

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