Abstract
Allergic conjunctivitis (AC), which is characterized by ocular itching, hyperemia, and edema, deteriorates quality of life. In this study, effects of anti-allergic drugs were evaluated by assessing eye-scratching behavior, the number of eosinophils in conjunctiva epithelial tissues, and concentrations of chemical mediators in the tears of the Guinea pig model of ovalbumin (OA)-induced AC. On day 0, 3-week-old Guinea pigs were sensitized by OA subconjunctival injections. On days 15, 17, and 19, OA solution was administered. Anti-allergic eye drops were administered 5 and 15 min before the final OA challenge on day 19. Scratching behavior within 1 h after OA exposure was studied. Eosinophils in the conjunctiva were stained with Giemsa reagent. Histamine and substance P (SP) concentrations in tears were measured using ELISA. Subconjunctivally injected Guinea pigs were observed for clinical symptoms. Scratching responses significantly reduced with ketotifen or olopatadine treatment. Eosinophil numbers reduced in animals treated with ketotifen, levocabastine, or tranilast. Histamine and/or SP concentrations in tears were inhibited by some of these anti-allergic drugs. It is important to assess the anti-allergic AC drugs objectively because there are several of these drugs currently available. This model allows for an objective evaluation of anti-allergic drugs for AC.
| Original language | English |
|---|---|
| Pages (from-to) | 231-238 |
| Number of pages | 8 |
| Journal | Open Medicine (Poland) |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Jan 1 |
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Keywords
- Allergic conjunctivitis
- Anti-allergic drugs
- Histamine
- Substance P
ASJC Scopus subject areas
- Medicine(all)
Cite this
Model for studying anti- allergic drugs for allergic conjunctivitis in animals. / Nakazawa, Yosuke; Oka, Mikako; Takehana, Makoto.
In: Open Medicine (Poland), Vol. 12, No. 1, 01.01.2017, p. 231-238.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Model for studying anti- allergic drugs for allergic conjunctivitis in animals
AU - Nakazawa, Yosuke
AU - Oka, Mikako
AU - Takehana, Makoto
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Allergic conjunctivitis (AC), which is characterized by ocular itching, hyperemia, and edema, deteriorates quality of life. In this study, effects of anti-allergic drugs were evaluated by assessing eye-scratching behavior, the number of eosinophils in conjunctiva epithelial tissues, and concentrations of chemical mediators in the tears of the Guinea pig model of ovalbumin (OA)-induced AC. On day 0, 3-week-old Guinea pigs were sensitized by OA subconjunctival injections. On days 15, 17, and 19, OA solution was administered. Anti-allergic eye drops were administered 5 and 15 min before the final OA challenge on day 19. Scratching behavior within 1 h after OA exposure was studied. Eosinophils in the conjunctiva were stained with Giemsa reagent. Histamine and substance P (SP) concentrations in tears were measured using ELISA. Subconjunctivally injected Guinea pigs were observed for clinical symptoms. Scratching responses significantly reduced with ketotifen or olopatadine treatment. Eosinophil numbers reduced in animals treated with ketotifen, levocabastine, or tranilast. Histamine and/or SP concentrations in tears were inhibited by some of these anti-allergic drugs. It is important to assess the anti-allergic AC drugs objectively because there are several of these drugs currently available. This model allows for an objective evaluation of anti-allergic drugs for AC.
AB - Allergic conjunctivitis (AC), which is characterized by ocular itching, hyperemia, and edema, deteriorates quality of life. In this study, effects of anti-allergic drugs were evaluated by assessing eye-scratching behavior, the number of eosinophils in conjunctiva epithelial tissues, and concentrations of chemical mediators in the tears of the Guinea pig model of ovalbumin (OA)-induced AC. On day 0, 3-week-old Guinea pigs were sensitized by OA subconjunctival injections. On days 15, 17, and 19, OA solution was administered. Anti-allergic eye drops were administered 5 and 15 min before the final OA challenge on day 19. Scratching behavior within 1 h after OA exposure was studied. Eosinophils in the conjunctiva were stained with Giemsa reagent. Histamine and substance P (SP) concentrations in tears were measured using ELISA. Subconjunctivally injected Guinea pigs were observed for clinical symptoms. Scratching responses significantly reduced with ketotifen or olopatadine treatment. Eosinophil numbers reduced in animals treated with ketotifen, levocabastine, or tranilast. Histamine and/or SP concentrations in tears were inhibited by some of these anti-allergic drugs. It is important to assess the anti-allergic AC drugs objectively because there are several of these drugs currently available. This model allows for an objective evaluation of anti-allergic drugs for AC.
KW - Allergic conjunctivitis
KW - Anti-allergic drugs
KW - Histamine
KW - Substance P
UR - http://www.scopus.com/inward/record.url?scp=85028046448&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028046448&partnerID=8YFLogxK
U2 - 10.1515/med-2017-0034
DO - 10.1515/med-2017-0034
M3 - Article
AN - SCOPUS:85028046448
VL - 12
SP - 231
EP - 238
JO - Open Medicine (Poland)
JF - Open Medicine (Poland)
SN - 2391-5463
IS - 1
ER -