Model of abnormal chromophore-protein interaction for E181K rhodopsin mutation

Computer molecular dynamics study

Tatyana Feldman, Mikhail Ostrovsky, Kholmirzo Kholmurodov, Kenji Yasuoka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The interaction of the 11-cis-retinal chromophore with the surrounding amino acid residues in the chromophore center of the rhodopsin protein has been investigated for the E181K mutant form using molecular dynamics simulation. A comparative analysis of the arrangement of the amino acid residues in the chromophore center has been performed for both wild (native) and mutant rhodopsins. It is shown that for the E181K mutant rhodopsin there is no proper binding of 11-cis-retinal with the surrounding amino acid residues. The distortion of the conformation states in the mutant rhodopsin molecule takes place in both the chromophore center and cytoplasmic domain. Our simulations suggest that a stable covalent linkage of 11-cis-retinal with the protein part (viz. opsin) of the rhodopsin molecule will not form. This, on the other hand, implies that the protein's active site in the cytoplasmic domain, which is responsible for the G-protein binding (so-called transducin), may not be completely blocked. Based on our molecular simulation data, we discuss the possible correlation between retinitis pigmentosa pathogenesis and the structural and functional properties of the rhodopsin protein.

Original languageEnglish
Pages (from-to)102
Number of pages1
JournalOpen Biochemistry Journal
Volume6
Publication statusPublished - 2012

Fingerprint

Rhodopsin
Molecular Dynamics Simulation
Chromophores
Molecular dynamics
Retinaldehyde
Mutation
Proteins
Amino Acids
Transducin
Opsins
Molecules
Retinitis Pigmentosa
GTP-Binding Proteins
Protein Binding
Conformations
Catalytic Domain
Computer simulation

Keywords

  • 11-cis-retinal chromophore
  • Molecular dynamics simulation
  • Mutant rhodopsin
  • Retinitis pigmentosa
  • Rhodopsin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Model of abnormal chromophore-protein interaction for E181K rhodopsin mutation : Computer molecular dynamics study. / Feldman, Tatyana; Ostrovsky, Mikhail; Kholmurodov, Kholmirzo; Yasuoka, Kenji.

In: Open Biochemistry Journal, Vol. 6, 2012, p. 102.

Research output: Contribution to journalArticle

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