Abstract
In order to elucidate the possible roles of various adhesion molecules in modifying the leukocyte behavior in the pulmonary microcirculation, we investigated, based on the isolated perfused lungs prepared from normal rats, the leukocyte kinetics in pulmonary arterioles, venules and capillaries under conditions in which functions of adhesion molecules were appropriately inhibited. Observation of leukocyte behavior and determination of microvascular architecture were precisely made by using a real-time confocal laser luminescence microscope. All the confocal images thus obtained were recorded on a high-speed and high-sensitivity video camera. The significant reduction in leukocyte velocity (i. e. leukocyte rolling) due to interfering interactions between the endothelium and the leukocyte was investigated in both arterioles and venules. However, the relative frequency of leukocytes with rolling was much higher in arterioles than that in venules Arteriolar rolling of unstimulated leukocytes was mediated via a L -selectin dependent mechanism, while venular rolling was governed by an ICAM-1 related mechanism. The entrapment of unstimulated leukocytes in capillaries was not conspicuously influenced by any adhesion molecules including ICAM-1 as well as P-and L-selectins. Stimulation of leukocytes with cytokine - induced neutrophil chemoattractant/gro (CINC/gro) which upregulates CD 11/CD 18 on the leukocyte surface significantly enhanced the numbers of rolling leukocytes in venules and of entrapped leukocytes in capillaries. These experimental results may suggest that leukocyte kinetics in the normal pulmonary microcirculation including arterioles, venules and capillaries is modified by different classes of adhesion molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 489-494 |
| Number of pages | 6 |
| Journal | Respiration and Circulation |
| Volume | 46 |
| Issue number | 5 |
| Publication status | Published - 1998 |
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Keywords
- Adhesion molecule
- Leukocyte kinetics
- Pulmonary microcirculation
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Cardiology and Cardiovascular Medicine
Cite this
Modification of Leukocyte Kinetics in Normal Pulmonary Microcirculation Caused by Adhesion Molecules. / Sato, Nagato; Suzuki, Yukio; Nishio, Kazumi; Aoki, Takuya; Suzuki, Koichi; Naoki, Katsuhiko; Kudo, Hiroyasu; Tsumura, Kuniharu; Yamaguchi, Kazuhiro.
In: Respiration and Circulation, Vol. 46, No. 5, 1998, p. 489-494.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modification of Leukocyte Kinetics in Normal Pulmonary Microcirculation Caused by Adhesion Molecules
AU - Sato, Nagato
AU - Suzuki, Yukio
AU - Nishio, Kazumi
AU - Aoki, Takuya
AU - Suzuki, Koichi
AU - Naoki, Katsuhiko
AU - Kudo, Hiroyasu
AU - Tsumura, Kuniharu
AU - Yamaguchi, Kazuhiro
PY - 1998
Y1 - 1998
N2 - In order to elucidate the possible roles of various adhesion molecules in modifying the leukocyte behavior in the pulmonary microcirculation, we investigated, based on the isolated perfused lungs prepared from normal rats, the leukocyte kinetics in pulmonary arterioles, venules and capillaries under conditions in which functions of adhesion molecules were appropriately inhibited. Observation of leukocyte behavior and determination of microvascular architecture were precisely made by using a real-time confocal laser luminescence microscope. All the confocal images thus obtained were recorded on a high-speed and high-sensitivity video camera. The significant reduction in leukocyte velocity (i. e. leukocyte rolling) due to interfering interactions between the endothelium and the leukocyte was investigated in both arterioles and venules. However, the relative frequency of leukocytes with rolling was much higher in arterioles than that in venules Arteriolar rolling of unstimulated leukocytes was mediated via a L -selectin dependent mechanism, while venular rolling was governed by an ICAM-1 related mechanism. The entrapment of unstimulated leukocytes in capillaries was not conspicuously influenced by any adhesion molecules including ICAM-1 as well as P-and L-selectins. Stimulation of leukocytes with cytokine - induced neutrophil chemoattractant/gro (CINC/gro) which upregulates CD 11/CD 18 on the leukocyte surface significantly enhanced the numbers of rolling leukocytes in venules and of entrapped leukocytes in capillaries. These experimental results may suggest that leukocyte kinetics in the normal pulmonary microcirculation including arterioles, venules and capillaries is modified by different classes of adhesion molecules.
AB - In order to elucidate the possible roles of various adhesion molecules in modifying the leukocyte behavior in the pulmonary microcirculation, we investigated, based on the isolated perfused lungs prepared from normal rats, the leukocyte kinetics in pulmonary arterioles, venules and capillaries under conditions in which functions of adhesion molecules were appropriately inhibited. Observation of leukocyte behavior and determination of microvascular architecture were precisely made by using a real-time confocal laser luminescence microscope. All the confocal images thus obtained were recorded on a high-speed and high-sensitivity video camera. The significant reduction in leukocyte velocity (i. e. leukocyte rolling) due to interfering interactions between the endothelium and the leukocyte was investigated in both arterioles and venules. However, the relative frequency of leukocytes with rolling was much higher in arterioles than that in venules Arteriolar rolling of unstimulated leukocytes was mediated via a L -selectin dependent mechanism, while venular rolling was governed by an ICAM-1 related mechanism. The entrapment of unstimulated leukocytes in capillaries was not conspicuously influenced by any adhesion molecules including ICAM-1 as well as P-and L-selectins. Stimulation of leukocytes with cytokine - induced neutrophil chemoattractant/gro (CINC/gro) which upregulates CD 11/CD 18 on the leukocyte surface significantly enhanced the numbers of rolling leukocytes in venules and of entrapped leukocytes in capillaries. These experimental results may suggest that leukocyte kinetics in the normal pulmonary microcirculation including arterioles, venules and capillaries is modified by different classes of adhesion molecules.
KW - Adhesion molecule
KW - Leukocyte kinetics
KW - Pulmonary microcirculation
UR - http://www.scopus.com/inward/record.url?scp=2442586893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442586893&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:2442586893
VL - 46
SP - 489
EP - 494
JO - Respiration and Circulation
JF - Respiration and Circulation
SN - 0452-3458
IS - 5
ER -