Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

K. Ohta, K. Shimazu, S. Komatsumoto, N. Araki, Mamoru Shibata, Y. Fukuuchi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effects of N(G)-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM N(G)-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM N(G)-monomethyl-L-arginine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (N(G),N(G)-dimethylarginine dimethyl- aminohydrolase).

Original languageEnglish
Pages (from-to)766-768
Number of pages3
JournalNeuroReport
Volume5
Issue number7
Publication statusPublished - 1994

Fingerprint

Corpus Striatum
Citrulline
Nitric Oxide Synthase
Arginine
omega-N-Methylarginine
Microdialysis
Aminohydrolases
NG-Nitroarginine Methyl Ester
Endothelium
Sprague Dawley Rats
Perfusion
Kidney
Enzymes

Keywords

  • Arginine
  • Citrulline
  • In vivo microdialysis
  • N(G),N(G)-dimethylarginine dimethyl-aminohydrolase
  • N(G)-monomethyl-L-arginine
  • N(G)-nitro-L- arginine methyl ester
  • Nitric oxide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ohta, K., Shimazu, K., Komatsumoto, S., Araki, N., Shibata, M., & Fukuuchi, Y. (1994). Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors. NeuroReport, 5(7), 766-768.

Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors. / Ohta, K.; Shimazu, K.; Komatsumoto, S.; Araki, N.; Shibata, Mamoru; Fukuuchi, Y.

In: NeuroReport, Vol. 5, No. 7, 1994, p. 766-768.

Research output: Contribution to journalArticle

Ohta, K, Shimazu, K, Komatsumoto, S, Araki, N, Shibata, M & Fukuuchi, Y 1994, 'Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors', NeuroReport, vol. 5, no. 7, pp. 766-768.
Ohta, K. ; Shimazu, K. ; Komatsumoto, S. ; Araki, N. ; Shibata, Mamoru ; Fukuuchi, Y. / Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors. In: NeuroReport. 1994 ; Vol. 5, No. 7. pp. 766-768.
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