Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

Norifumi Kawakami; Yosuke Hara; Kenji Miyamoto

doi:10.1039/c5cy00679a

Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

Norifumi Kawakami, Yosuke Hara, Kenji Miyamoto

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min<sup>-1</sup> by the addition of 1,3-dioxolane, which altered the substrate specificity.

Original language	English
Pages (from-to)	3922-3925
Number of pages	4
Journal	Catalysis Science and Technology
Volume	5
Issue number	8
DOIs	https://doi.org/10.1039/c5cy00679a
Publication status	Published - 2015 Aug 1

ASJC Scopus subject areas

Catalysis

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abstract = "The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.",

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