Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

Norifumi Kawakami; Yosuke Hara; Kenji Miyamoto

doi:10.1039/c5cy00679a

Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

Norifumi Kawakami, Yosuke Hara, Kenji Miyamoto

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min<sup>-1</sup> by the addition of 1,3-dioxolane, which altered the substrate specificity.

Original language	English
Pages (from-to)	3922-3925
Number of pages	4
Journal	Catalysis Science and Technology
Volume	5
Issue number	8
DOIs	https://doi.org/10.1039/c5cy00679a
Publication status	Published - 2015 Aug 1

ASJC Scopus subject areas

Catalysis

Access to Document

10.1039/c5cy00679a

Cite this

@article{ca0bbaff19604c8e999cbc6fc46d2850,

title = "Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers",

abstract = "The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.",

author = "Norifumi Kawakami and Yosuke Hara and Kenji Miyamoto",

year = "2015",

month = aug,

day = "1",

doi = "10.1039/c5cy00679a",

language = "English",

volume = "5",

pages = "3922--3925",

journal = "Catalysis Science and Technology",

issn = "2044-4753",

publisher = "Royal Society of Chemistry",

number = "8",

}

TY - JOUR

T1 - Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

AU - Kawakami, Norifumi

AU - Hara, Yosuke

AU - Miyamoto, Kenji

PY - 2015/8/1

Y1 - 2015/8/1

N2 - The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.

AB - The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.

UR - http://www.scopus.com/inward/record.url?scp=84937428500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937428500&partnerID=8YFLogxK

U2 - 10.1039/c5cy00679a

DO - 10.1039/c5cy00679a

M3 - Article

AN - SCOPUS:84937428500

VL - 5

SP - 3922

EP - 3925

JO - Catalysis Science and Technology

JF - Catalysis Science and Technology

SN - 2044-4753

IS - 8

ER -

Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this