Abstract
The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min<sup>-1</sup> by the addition of 1,3-dioxolane, which altered the substrate specificity.
| Original language | English |
|---|---|
| Pages (from-to) | 3922-3925 |
| Number of pages | 4 |
| Journal | Catalysis Science and Technology |
| Volume | 5 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2015 Aug 1 |
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ASJC Scopus subject areas
- Catalysis
Cite this
Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers. / Kawakami, Norifumi; Hara, Yosuke; Miyamoto, Kenji.
In: Catalysis Science and Technology, Vol. 5, No. 8, 01.08.2015, p. 3922-3925.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulating the catalytic activity and the substrate specificity of alcohol dehydrogenases using cyclic ethers
AU - Kawakami, Norifumi
AU - Hara, Yosuke
AU - Miyamoto, Kenji
PY - 2015/8/1
Y1 - 2015/8/1
N2 - The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.
AB - The catalytic activities of alcohol dehydrogenases (ADHs) are investigated in the presence of 5% cyclic ethers. Although most cyclic ethers show an inhibitory effect on ADHs, we found that the catalytic rate constant of 2-butanol oxidation by Thermoanaerobacter brockii ADH was increased from 57 to 190 min-1 by the addition of 1,3-dioxolane, which altered the substrate specificity.
UR - http://www.scopus.com/inward/record.url?scp=84937428500&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937428500&partnerID=8YFLogxK
U2 - 10.1039/c5cy00679a
DO - 10.1039/c5cy00679a
M3 - Article
AN - SCOPUS:84937428500
VL - 5
SP - 3922
EP - 3925
JO - Catalysis Science and Technology
JF - Catalysis Science and Technology
SN - 2044-4753
IS - 8
ER -