Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo

R. Takamiya, S. Hayashi, N. Goda, Y. Wakabayashi, M. Kitajima, T. Tamatani, S. Tojo, Y. Ishimura, M. Suematsu

Research output: Contribution to journalArticle

Abstract

This study aimed to examine whether induction of heme oxygenase-1 limits endothelial cell-leukocyte interactions in vivo. Leukocyte traffics were visually monitored in rat mesenteric post-capillary venules under monitoring regional erythrocyte velocity using intravital video microscopy. In the control rats, rolling and stationally adhesion of leukocytes in venular endothelium were elicited in response to superfusion with 500μM H 2O 2 without showing a reduction of shear rates. These changes were abolished by pretreatment with an anti-rat P-selectin MoAb ARP2.4. In addition, laser confocal microfluorography assisted by fluorescence-labelled ARP2.4 revealed that the H 2O 2 superfusion enhanced the P-selectin expression in venules. When rats were pretreated for 12 hrs with an intraperitoneal injection of hemin, microvascular endothelium as well as parenchymal cellular components including mast cells displayed a marked expression of HO-1. Under these circumstances, the H 2O 2-elicited endothelial cell-leukocyte interactions were attenuated almost completely. On the other hand, superfusion with 0.1 μM zinc protoporphyrin IX, an inhibitor of HO, restored the H 2O 2-elicited P-selectin expression and subsequent adhesive responses of leukocytes. The present findings thus suggest that HO-1 induction modulates endothelium-leukocyte interaction in vivo.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - 1998 Mar 20

Fingerprint

Heme Oxygenase (Decyclizing)
P-Selectin
Venules
Endothelial cells
heme
oxygenases
Cell Communication
endothelial cells
Rats
leukocytes
Leukocytes
Endothelial Cells
Modulation
rats
endothelium
Rat control
Endothelium
Hemin
Heme Oxygenase-1
Shear deformation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Takamiya, R., Hayashi, S., Goda, N., Wakabayashi, Y., Kitajima, M., Tamatani, T., ... Suematsu, M. (1998). Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo. FASEB Journal, 12(5).

Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo. / Takamiya, R.; Hayashi, S.; Goda, N.; Wakabayashi, Y.; Kitajima, M.; Tamatani, T.; Tojo, S.; Ishimura, Y.; Suematsu, M.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Takamiya, R, Hayashi, S, Goda, N, Wakabayashi, Y, Kitajima, M, Tamatani, T, Tojo, S, Ishimura, Y & Suematsu, M 1998, 'Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo', FASEB Journal, vol. 12, no. 5.
Takamiya, R. ; Hayashi, S. ; Goda, N. ; Wakabayashi, Y. ; Kitajima, M. ; Tamatani, T. ; Tojo, S. ; Ishimura, Y. ; Suematsu, M. / Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
@article{65f21f1147f244b3a620df149b5791a3,
title = "Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo",
abstract = "This study aimed to examine whether induction of heme oxygenase-1 limits endothelial cell-leukocyte interactions in vivo. Leukocyte traffics were visually monitored in rat mesenteric post-capillary venules under monitoring regional erythrocyte velocity using intravital video microscopy. In the control rats, rolling and stationally adhesion of leukocytes in venular endothelium were elicited in response to superfusion with 500μM H 2O 2 without showing a reduction of shear rates. These changes were abolished by pretreatment with an anti-rat P-selectin MoAb ARP2.4. In addition, laser confocal microfluorography assisted by fluorescence-labelled ARP2.4 revealed that the H 2O 2 superfusion enhanced the P-selectin expression in venules. When rats were pretreated for 12 hrs with an intraperitoneal injection of hemin, microvascular endothelium as well as parenchymal cellular components including mast cells displayed a marked expression of HO-1. Under these circumstances, the H 2O 2-elicited endothelial cell-leukocyte interactions were attenuated almost completely. On the other hand, superfusion with 0.1 μM zinc protoporphyrin IX, an inhibitor of HO, restored the H 2O 2-elicited P-selectin expression and subsequent adhesive responses of leukocytes. The present findings thus suggest that HO-1 induction modulates endothelium-leukocyte interaction in vivo.",
author = "R. Takamiya and S. Hayashi and N. Goda and Y. Wakabayashi and M. Kitajima and T. Tamatani and S. Tojo and Y. Ishimura and M. Suematsu",
year = "1998",
month = "3",
day = "20",
language = "English",
volume = "12",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "5",

}

TY - JOUR

T1 - Modulation of P-selectin dependent leukocyte-endothelial cell interactions by inducible heme oxygenase in rat post-capillary venules in vivo

AU - Takamiya, R.

AU - Hayashi, S.

AU - Goda, N.

AU - Wakabayashi, Y.

AU - Kitajima, M.

AU - Tamatani, T.

AU - Tojo, S.

AU - Ishimura, Y.

AU - Suematsu, M.

PY - 1998/3/20

Y1 - 1998/3/20

N2 - This study aimed to examine whether induction of heme oxygenase-1 limits endothelial cell-leukocyte interactions in vivo. Leukocyte traffics were visually monitored in rat mesenteric post-capillary venules under monitoring regional erythrocyte velocity using intravital video microscopy. In the control rats, rolling and stationally adhesion of leukocytes in venular endothelium were elicited in response to superfusion with 500μM H 2O 2 without showing a reduction of shear rates. These changes were abolished by pretreatment with an anti-rat P-selectin MoAb ARP2.4. In addition, laser confocal microfluorography assisted by fluorescence-labelled ARP2.4 revealed that the H 2O 2 superfusion enhanced the P-selectin expression in venules. When rats were pretreated for 12 hrs with an intraperitoneal injection of hemin, microvascular endothelium as well as parenchymal cellular components including mast cells displayed a marked expression of HO-1. Under these circumstances, the H 2O 2-elicited endothelial cell-leukocyte interactions were attenuated almost completely. On the other hand, superfusion with 0.1 μM zinc protoporphyrin IX, an inhibitor of HO, restored the H 2O 2-elicited P-selectin expression and subsequent adhesive responses of leukocytes. The present findings thus suggest that HO-1 induction modulates endothelium-leukocyte interaction in vivo.

AB - This study aimed to examine whether induction of heme oxygenase-1 limits endothelial cell-leukocyte interactions in vivo. Leukocyte traffics were visually monitored in rat mesenteric post-capillary venules under monitoring regional erythrocyte velocity using intravital video microscopy. In the control rats, rolling and stationally adhesion of leukocytes in venular endothelium were elicited in response to superfusion with 500μM H 2O 2 without showing a reduction of shear rates. These changes were abolished by pretreatment with an anti-rat P-selectin MoAb ARP2.4. In addition, laser confocal microfluorography assisted by fluorescence-labelled ARP2.4 revealed that the H 2O 2 superfusion enhanced the P-selectin expression in venules. When rats were pretreated for 12 hrs with an intraperitoneal injection of hemin, microvascular endothelium as well as parenchymal cellular components including mast cells displayed a marked expression of HO-1. Under these circumstances, the H 2O 2-elicited endothelial cell-leukocyte interactions were attenuated almost completely. On the other hand, superfusion with 0.1 μM zinc protoporphyrin IX, an inhibitor of HO, restored the H 2O 2-elicited P-selectin expression and subsequent adhesive responses of leukocytes. The present findings thus suggest that HO-1 induction modulates endothelium-leukocyte interaction in vivo.

UR - http://www.scopus.com/inward/record.url?scp=33749278460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749278460&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749278460

VL - 12

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 5

ER -