Molecular analysis of Dirofilaria repens removed from a subcutaneous nodule in a Japanese woman after a tour to Europe

Jun Suzuki, Seiki Kobayashi, Utako Okata, Hitomi Matsuzaki, Mariko Mori, Ko Ron Chen, Satoshi Iwata

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A premature female Dirofilaria species, subsequently identified as Dirofilaria repens by its morphological features and mitochondrial 12S ribosomal RNA (12S rRNA) gene sequence, was removed from a subcutaneous nodule of the right temporal region of the head in a Japanese woman 2 years after she noticed swelling of her left calf following an insect sting during a tour to Europe; headache symptoms were noticed a few months later. The sequences of the mitochondrial 12S rRNA and cytochrome c oxidase subunit I genes from the organism were almost identical to those of sequences AM779772 (100% homology, 337/337) and AM749233 (99.8% homology, 536/537) of D. repens isolated from humans in Italy. However, the phylogenetic position of the 18S rRNA-internal transcribed spacer 1-5.8S rRNA region was in the same cluster as that of sequence JX290195 of Dirofilaria sp. "hongkongensis" (96.7% homology, 348/360), which was recently reported from Hong Kong as a novel Dirofilaria species. Information on regional genetic variation in D. repens isolated from animals and humans remains scarce. We report the detailed genetic features of this filaria as a reference isolate from a specific endemic area, to enrich the genetic database of D. repens.

Original languageEnglish
Pages (from-to)2
Number of pages1
JournalParasite
Volume22
DOIs
Publication statusPublished - 2015

Fingerprint

Dirofilaria repens
Dirofilaria
ribosomal RNA
Insect Bites and Stings
Genetic Databases
headache
Hong Kong
Electron Transport Complex IV
Temporal Lobe
cytochrome-c oxidase
Italy
internal transcribed spacers
Genes
signs and symptoms (animals and humans)
Headache
Head
calves
nucleotide sequences
genetic variation
insects

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular analysis of Dirofilaria repens removed from a subcutaneous nodule in a Japanese woman after a tour to Europe. / Suzuki, Jun; Kobayashi, Seiki; Okata, Utako; Matsuzaki, Hitomi; Mori, Mariko; Chen, Ko Ron; Iwata, Satoshi.

In: Parasite, Vol. 22, 2015, p. 2.

Research output: Contribution to journalArticle

Suzuki, Jun ; Kobayashi, Seiki ; Okata, Utako ; Matsuzaki, Hitomi ; Mori, Mariko ; Chen, Ko Ron ; Iwata, Satoshi. / Molecular analysis of Dirofilaria repens removed from a subcutaneous nodule in a Japanese woman after a tour to Europe. In: Parasite. 2015 ; Vol. 22. pp. 2.
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