Molecular and morphological analysis of an Antarctic tardigrade, Acutuncus antarcticus

Hiroshi Kagoshima, Satoshi Imura, Atsushi C. Suzuki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We isolated a species of tardigrade from moss samples collected from Langhovde and Skarvsnes, near Syowa station, East Antarctic, from which we cultured a parthenogenetic strain in Petri dishes with co-occurring cyanobacteria or green algae. This culture was maintained at both 4 and 10°C, though the latter proved more suitable for growth. Eggs were laid free, rather than in exuviae. We isolated the 18S rRNA sequences from this tardigrade, identical to that ofacutuncus antarcticusfrom King George island, South Shetland islands. Morphological analyses via both light and scanning electron microscopy also show general agreement with characteristics of a. antarcticus: dorsal and ventral apophyses for the insertion of stylet muscles and dorsal longitudinal thickening on the anterior part of buccal tube; presence of pharyngeal apophyses, two macroplacoids and absence of a microplacoid; the surface structure of egg; and claw shape. Peribuccal lamellae were absent, but six oval swellings surrounded the mouth opening. An additional study of moss pillars from lake Hotoke-ike, Skarvsnes, proved the existence of the same tardigrade taxon living at the bottom of the lake.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalJournal of Limnology
Volume72
Issue numberSUPPL 1
DOIs
Publication statusPublished - 2013 Aug 2

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Keywords

  • 18S rRNA
  • Antarctica
  • Cultivation
  • Exuviae
  • JARE (Japanese Antarctic Research Expedition)
  • Tardigrada

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology
  • Water Science and Technology

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