Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions

Tetsuro Kobayashi, Akiko Fujisawa, Masayuki Amagai, Toshiroh Iwasaki, Manabu Ohyama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The dermal papilla (DP) plays pivotal roles in hair follicle morphogenesis and cycling. However, our understanding of the biology of the canine DP is extremely limited. The aim of this study was to elucidate molecular biological and immunohistochemical characteristics of canine DP cells and determine appropriate conditions for in vitro expansion. Histological investigation revealed that the canine DP expressed biomarkers of human and rodent DP, including alkaline phosphatase (ALP) and versican. When microdissected, canine DP, but not fibroblasts, strongly expressed the DP-related genes for alkaline phosphatase, Wnt inhibitory factor1 and lymphoid enhancer-binding factor1, confirming successful isolation. The growth rate of isolated canine DP cells was moderate in conventional culture conditions for rodent and human DP; however, AmnioMAX-C100 complete medium allowed more efficient cultivation. Dermal papilla marker gene expression was maintained in early passage cultured DP cells, but gradually lost after the third passage. Approaches to mimic the in vivo DP environment in culture, such as supplementation of keratinocyte-conditioned medium or use of extracellular matrix-coated dishes, moderately ameliorated loss of DP gene expression in canine DP cells. It is possible that constituent factors in AmnioMAX may influence culture. These findings suggested that further refinements of culture conditions may enable DP cell expansion without impairing intrinsic properties and, importantly, demonstrated that AmnioMAX-cultured early passage canine DP cells partly maintained the biological characteristics of in vivo canine DP cells. This study provides crucial information necessary for further optimization of culture conditions of canine DP.

Original languageEnglish
Pages (from-to)414-422
Number of pages9
JournalVeterinary Dermatology
Volume22
Issue number5
DOIs
Publication statusPublished - 2011 Oct

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Canidae
Cell Culture Techniques
Skin
dogs
cells
alkaline phosphatase
rodents
gene expression
hair follicles
keratinocytes
extracellular matrix
morphogenesis
fibroblasts
Alkaline Phosphatase
Rodentia
biomarkers
Versicans
Gene Expression
Biological Sciences
genetic markers

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions. / Kobayashi, Tetsuro; Fujisawa, Akiko; Amagai, Masayuki; Iwasaki, Toshiroh; Ohyama, Manabu.

In: Veterinary Dermatology, Vol. 22, No. 5, 10.2011, p. 414-422.

Research output: Contribution to journalArticle

Kobayashi, Tetsuro ; Fujisawa, Akiko ; Amagai, Masayuki ; Iwasaki, Toshiroh ; Ohyama, Manabu. / Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of culture conditions. In: Veterinary Dermatology. 2011 ; Vol. 22, No. 5. pp. 414-422.
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