Molecular biology of the hst-1 gene.

T. Sugimura, T. Yoshida, H. Sakamoto, O. Katoh, Yutaka Hattori, M. Terada

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Abstract

The hst-1 gene (or HSTF1 by human gene nomenclature) was originally identified in our laboratory by an NIH/3T3 focus formation assay using DNA from a human gastric cancer. Sequence analysis predicted the hst-1 product to be a novel growth factor with 30-50% homology with six other heparin-binding growth factors: basic and acidic fibroblast growth factors (FGFs), the int-2 protein, FGF5, the hst-2/FGF6 protein and keratinocyte growth factor (KGF). A recombinant hst-1 protein was synthesized in silkworm cells and found to be a potent heparin-binding mitogen for murine fibroblasts and human vascular endothelial cells. Although hst-1 expression cannot be detected in most cancer cells, including gastric cancers, it is expressed in mouse embryos and in some germ cell tumours. Both hst-1 and int-2 are located on band q13.3 of human chromosome 11 within a distance of 35 kbp; in the mouse genome these two genes are separated by less than 20 kbp. They are differentially transcribed in the F9 mouse teratocarcinoma cell line; hst-1 is expressed in undifferentiated stem cells and int-2 in differentiated endodermal cells. The hst-1 and int-2 genes were coamplified in a variety of cancer cells, most notably in more than 50% of oesophageal cancers.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalCiba Foundation symposium
Volume150
Publication statusPublished - 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

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Sugimura, T., Yoshida, T., Sakamoto, H., Katoh, O., Hattori, Y., & Terada, M. (1990). Molecular biology of the hst-1 gene. Ciba Foundation symposium, 150, 79-89.