Molecular characterization of the mouse vesicular acetylcholine transporter gene

Jorge M. Naciff, Hidemi Misawa, John R. Dedman

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The organization of the mouse choline acetyltransferase (ChAT) gene has been previously analyzed. Here we show that the first intron of the mouse ChAT gene contains an uninterrupted open reading frame. It is in the same transcriptional orientation as ChAT and encodes the vesicular acetylcholine (ACh) transporter (VAChT), the protein responsible for the translocation of cytoplasmic ACh into synaptic vesicles. The sequence of this transporter is very similar to the VAChT from rat and human (99% and 95% identity, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed expression of mouse VAChT mRNA in spinal cord, brain (excluding the cerebellum) and brain stem, but not in peripheral tissues such as liver and kidney. Transgenic mouse analysis revealed that the 5'-flanking region of the mouse ChAT gene encompasses regulatory elements that allowed elevated expression of VAChT in the cholinergic system of transgenic mice.

Original languageEnglish
Pages (from-to)3467-3473
Number of pages7
JournalNeuroReport
Volume8
Issue number16
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Vesicular Acetylcholine Transport Proteins
Choline O-Acetyltransferase
Transgenic Mice
Genes
Synaptic Vesicles
5' Flanking Region
Protein Transport
Regulator Genes
Introns
Cerebellum
Cholinergic Agents
Open Reading Frames
Brain Stem
Reverse Transcription
Acetylcholine
Spinal Cord
Kidney
Polymerase Chain Reaction
Messenger RNA
Liver

Keywords

  • 5'-Flanking region of the mouse choline acetyltransferase gene
  • Molecular cloning
  • Mouse
  • Mouse cholinergic system
  • Vesicular acetylcholine transporter gene

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Molecular characterization of the mouse vesicular acetylcholine transporter gene. / Naciff, Jorge M.; Misawa, Hidemi; Dedman, John R.

In: NeuroReport, Vol. 8, No. 16, 1997, p. 3467-3473.

Research output: Contribution to journalArticle

Naciff, Jorge M. ; Misawa, Hidemi ; Dedman, John R. / Molecular characterization of the mouse vesicular acetylcholine transporter gene. In: NeuroReport. 1997 ; Vol. 8, No. 16. pp. 3467-3473.
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