Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens

Fereshteh Shahcheraghi, Yusuke Minato, Jing Chen, Tohru Mizushima, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We cloned a gene smfY for multidrug efflux pump from chromosomal DNA of Serratia marcescens using drug-hypersensitive Escherichia coli KAM32 as the host, and characterized the pump. E. coli KAM32/pESM42 carrying the smfY showed significantly increased MICs of various drugs including DAPI, norfloxacin, benzalkonium chloride, acriflavine and ethidium bromide, compared with the control. We also detected energy-dependent ethidium and acriflavine efflux due to the SmfY. Sequence analysis revealed that the SmfY was a multidrug ef-flux pump of the MF (Major Facilitator) superfamily transporters. This is the first report of a multidrug efflux pump belonging to the MF superfamily in S. marcescens.

Original languageEnglish
Pages (from-to)798-800
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number4
DOIs
Publication statusPublished - 2007 Apr 1

Keywords

  • Major facilitator (MF) superfamily
  • Multidrug efflux pump
  • Serratia marcescens
  • SmfY

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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    Shahcheraghi, F., Minato, Y., Chen, J., Mizushima, T., Ogawa, W., Kuroda, T., & Tsuchiya, T. (2007). Molecular cloning and characterization of a multidrug efflux pump, SmfY, from Serratia marcescens. Biological and Pharmaceutical Bulletin, 30(4), 798-800. https://doi.org/10.1248/bpb.30.798