Molecular cloning and characterization of annexin V-binding proteins with highly hydrophilic peptide structure

Keiko Ohsawa, Yoshinori Imai, Daisuke Ito, Shinichi Kohsaka

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30 Citations (Scopus)

Abstract

We previously reported that annexin V promoted the survival of cultured rat neocortical neurons. In an effort to elucidate the mechanism underlying this neurotrophic activity of annexin V, we have attempted to identify the target or binding proteins of annexin V in neuronal cells. Herein, we screened an embryonic day 17 rat brain cDNA library by western blot using glutathione S-transferase-annexin V fusion protein as a probe and then isolated four clones showing binding to annexin V in a Ca2+- and phospholipid-dependent manner. Although these cDNAs encoded different polypeptides, all four polypeptides shared the unique feature of containing highly hydrophilic stretches with high Lys, Glu, and Ser contents. Deduced amino acid sequences of two clones showed high homology with human X-linked Helicase2 (XH2) and DNA (cytosine-5) methyltransferase (DMTase) sequences, whereas the other two were not related to any known peptide sequence. These results suggest that XH2 and DMTase are candidates for annexin V-binding proteins and thus may mediate the biological activity of annexin V.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalJournal of Neurochemistry
Volume67
Issue number1
Publication statusPublished - 1996 Jul
Externally publishedYes

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Keywords

  • Annexin V
  • Binding protein
  • DNA methyltransferase
  • Helicase
  • Neuron

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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