TY - JOUR
T1 - Molecular cloning and characterization of growth hormone receptor and its homologue in the Japanese eel (Anguilla japonica)
AU - Ozaki, Yuichi
AU - Fukada, Haruhisa
AU - Kazeto, Yukinori
AU - Adachi, Shinji
AU - Hara, Akihiko
AU - Yamauchi, Kohei
N1 - Funding Information:
We thank Dr. Rüdiger W. Schulz (University of Utrecht, The Netherlands) for critical reading of the manuscript, and our colleagues in the eel research group for their encouragement. This study was supported by grants from the Fisheries Agency, and from the Japan Society for the Promotion of Science, and from the 21st Century COE program “Marine Bio-Manipulation Frontier for Food Production” of the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2006/4
Y1 - 2006/4
N2 - Two cDNAs encoding growth hormone receptor (GHR)-like genes, eGHR1 and eGHR2, were isolated from Japanese eel (Anguilla japonica) liver tissue. The putative eel GHR proteins showed conserved structural features of vertebrate GHRs, including six cysteine residues and a YGEFS motif in the extracellular domain, a single transmembrane region, and proline-rich box 1 and box 2 domains. Northern blot analysis showed a single eGHR1 transcript in liver, while two sizes of eGHR2 transcripts, thought to be produced by alternative splicing, were present. RT-PCR revealed that eGHR1 and eGHR2 transcripts were widely distributed throughout the whole body of the Japanese eel. Moreover, the results of binding assays showed the specific binding of growth hormone to recombinant eGHR1. Since these putative eGHR proteins show all characteristics of the GHR family, we conclude that eGHR1 and eGHR2 cDNA encode two different GHRs in Japanese eel. We confirmed the ligand specificity of eGHR1 by binding assay, and further research is needed to allow characterization of the binding capability of eGHR2.
AB - Two cDNAs encoding growth hormone receptor (GHR)-like genes, eGHR1 and eGHR2, were isolated from Japanese eel (Anguilla japonica) liver tissue. The putative eel GHR proteins showed conserved structural features of vertebrate GHRs, including six cysteine residues and a YGEFS motif in the extracellular domain, a single transmembrane region, and proline-rich box 1 and box 2 domains. Northern blot analysis showed a single eGHR1 transcript in liver, while two sizes of eGHR2 transcripts, thought to be produced by alternative splicing, were present. RT-PCR revealed that eGHR1 and eGHR2 transcripts were widely distributed throughout the whole body of the Japanese eel. Moreover, the results of binding assays showed the specific binding of growth hormone to recombinant eGHR1. Since these putative eGHR proteins show all characteristics of the GHR family, we conclude that eGHR1 and eGHR2 cDNA encode two different GHRs in Japanese eel. We confirmed the ligand specificity of eGHR1 by binding assay, and further research is needed to allow characterization of the binding capability of eGHR2.
KW - Binding assay
KW - Cloning
KW - Cytokine receptor family
KW - Dig
KW - Growth hormone
KW - Growth hormone receptor
KW - Japanese eel
KW - Prolactin
KW - Somatolactin
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U2 - 10.1016/j.cbpb.2005.12.016
DO - 10.1016/j.cbpb.2005.12.016
M3 - Article
C2 - 16458558
AN - SCOPUS:33644895112
SN - 1096-4959
VL - 143
SP - 422
EP - 431
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 4
ER -