Molecular cloning and characterization of mouse aquaporin 6

Hiroaki Nagase, Johan Ågren, Akiko Saito, Kun Liu, Peter Agre, Akihiro Hazama, Masato Yasui

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the rat kidney, aquaporin (AQP) 6 is localized in the intracellular vesicle membranes of type-A intercalated cells of the collecting duct; mouse AQP6 (mAQP6) has not been characterized. Although mAQP6 was originally cloned from cDNA in a mouse cerebellum library (GenBank NM 175087), we have independently cloned a cDNA encoding mAQP6 from an adult kidney cDNA library (C57BL/6J strain). We identified two different spliced variants of mAQP6: mAQP6a and mAQP6b. The mAQP6a isoform is almost identical to that of rat AQP6, whereas mAQP6b is identical to that reported in the mouse cerebellum library mentioned above. We found that the mRNA expression of these two spliced variants is regulated in a tissue-specific and age-dependent manner. Functional analyses of water and ion permeation revealed that mAQP6a functions like rat AQP6 and that mAQP6b does not function as either a water channel or an ion channel under our experimental conditions.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume352
Issue number1
DOIs
Publication statusPublished - 2007 Jan 5

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Keywords

  • Aquaporin
  • Splice variant

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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